不稳定性心绞痛患者血管紧张素转换酶基因多态性与血脂水平的相关性研究

Gene Polymorphism and Serum Lipid Levels Correlation of Unstable Angina Angiotensin Converting Enzyme

目的:探讨老年不稳定性心绞痛(UA)患者ACE基因多态性与血脂水平的相关性,为中医药治疗本病提供参考。方法:选取老年不稳定性心绞痛患者60例,同期选择老年健康人群30例作为对照,通过限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术测定ACE基因内含子16的插入和缺失多态性,并比较不同基因型的血脂水平差异。结果:老年UA组ACE基因内含子16携带DD基因型和D等位基因较对照组增高,对照组携带II基因型及I等位基因较老年UA组增高(P<0.05)。老年UA组中TG、LDL-C血浓度较对照组增高,疾病组TG、LDL-C水平明显高于对照组(P<0.05),而HDL-C水平明显低于对照组(P<0.05)。且疾病组中ACE DD、ID基因型者与II型者间相比差异有统计学意义(P<0.05);ACE基因多态性与TG、LDL-C间呈显著正相关(P值均<0.01);与HDL-C浓度间存在显著负相关(P<0.001)。结论:ACE基因DD型和D等位基因与血脂代谢异常有关,而与I等位基因无关。

Objective：Discussion the correlation of ACE gene polymorphism and serum lipid levels on the elderly unstable angina（ UA）, as the traditional Chinese medicine treatment of the disease to provide reference. Methods：Choose 60 cases of elderly patients with un-stable angina,with 30 cases of healthy people as a control. Through the restriction fragment length polymorphism by polymerase chain reaction（ PCR-RFLP）to determine the insertions and deletions included 16 sub-polymorphism and serum lipid levels of ACE gene and compare the different genotypes. Results：The intron of ACE gene in UA group carrying DD genotype and D allele was higher than that of control group. The control group carrying II genotype and I allele was higher than that of old UA group（P〈0. 05）. The TG and LDL-C blood concentrations of Elderly UA group were higher than that of control group. The TG and LDL-C levels of disease group were sig-nificantly higher（P〈0. 05）,whereas HDL-C levels were significantly lower than that of control group（P〈0. 05）. Compared the ACE DD and ID genotype of disease groups with II genotype,the difference had statistical significance（P〈0. 05）. ACE gene polymorphism and TG,LDL-C had significantly positively correlated（P〈0. 01）but negative correlation with HDL-C concentrations（P〈0. 001）. Con-clusion：ACE DD genotype and D allele have relation with abnormal lipid metabolism,but have nothing to do with I allele.