白芍总苷对NAFLD大鼠肝脏的保护作用及其机制研究

Protection effect and its mechanism of total glucosides of paeony on the fatty liver in NAFLD rats

目的研究白芍总苷对NAFLD模型大鼠肝脏保护的机制。方法 SD大鼠50只,随机分为正常对照组10只,模型组40只。高脂饲料喂养建模后,模型组随机分为模型对照组、非诺贝特组、白芍总苷高剂量组、白芍总苷低剂量组各10只。连续灌胃给药4周后,测定大鼠血清中ALT、AST、GST、SOD和GSH-PX酶的活性及MDA含量;测定血清中IL-1、TNF-α和TGF-β的水平;各组取肝脏做HE染色,观察肝脏组织学病变。结果与正常对照组比较,NAFLD模型对照组大鼠ALT、AST、GST活性和IL-1、TNF-α、TGF-β含量显著升高,经非诺贝特和白芍总苷干预后,模型组大鼠的ALT、AST、GST活性和IL-1、TNF-α、TGF-β含量均降低回正常水平,差异有统计学意义(P<0.05);与正常对照组比较,NALFD模型大鼠SOD、GSH-PX活性显著降低,MDA含量明显升高,干预治疗后,模型组大鼠SOD、GSH-PX活性大大提高,MDA含量显著降低(P<0.05)。结论白芍总苷对高脂引起的NAFLD模型大鼠有肝脏保护作用,其作用机制可能与其提高抗氧化能力、降低异常细胞因子水平有关。

Objective To explore the protection mechanism of total glucosides of paeony （TGP）on fatty lver of NAFLD rats.Methods 50 rats were randomly divided into control group （n =10） and model group （n =40）,model group were fed with high-fat diet and then were randomly divided into 4 groups,including nonalcoholic fatty liver group,fenofibrate group,high-dose TGP group and low-dose TGP group.After four weeks lavage,the activity of ALT,AST,GST,SOD,GSH-PX and the content of IL-1,TNF-α,TGF-β were determined.Each group was extracted liver for HE staining,to observe the histologic lesions.Results Compared with normal control group,the activities of serum ALT,AST,GST and the content of IL-1,TNF-α,TGF-β increased in model control group （P ＜ 0.05）.After treatment with TGP and fenofibrate,the activities of serum ALT,AST,GST and the content of IL-1,TNF-α,TGF-β significantly decreased（P ＜ 0.05）.Meantime,the activities of SOD,GSH-PX decreased in model rats and MDA content increased significantly.After treatment,activities of SOD,GSH-PX in model group rats greatly increased,MDA content decreased significantly （P ＜ 0.05）.Conclusion TGP has protective effect on NAFLD model rats,the mechanism may be related to improving the antioxidant ability and reducing abnormal cytokine levels.