摘要
目的:通过生物芯片技术观察茶多酚表没食子儿茶素没食子酸酯(EGCG)对Hep G2细胞脂质代谢相关基因和长链非编码RNA(lnc RNAs)表达的影响并探讨后两者可能的作用关系。方法 :人HepG 2细胞经EGCG(25μmol/L)处理24 h后,抽提RNA,并杂交至Human Transcriptome Array 2.0芯片进行mR NA和lncR NAs表达谱的分析。通过生物信息学进行靶基因预测建立两者的可能作用关系,并应用实时荧光定量多聚酶链反应(PCR)技术对芯片结果验证。结果 :HepG 2细胞经EGCG处理后表达差异的脂质代谢相关基因为27条,其中11条涉及胆固醇代谢。此外,芯片结果显示有285条lncR NAs表达上调或下调。靶基因预测发现多个脂代谢基因可能受lncR NAs顺式或反式调控,实时定量PCR验证与芯片结果一致。结论 :茶多酚EGCG可直接改善脂质代谢包括胆固醇的代谢,lncR NAs可能参与了对脂质代谢基因的表达调控。
Objective： To investigate the effects of epigallocatechingallate（EGCG） on lipid metabolism related gene and long noncoding RNA（lnc RNA） expression profile by biochip technology, and to explore the possible relationship between the two elements.Methods： HepG 2 cell was cultured with EGCG at 25μ mol/L for 24 hours, the total RNA was extracted and hybridized into the biochip of Human Transcriptome Array 2.0 for m RNA and lnc RNA expression profile analysis. Bioinformatics technology was used to establish the possible relationship between lncR NA and the predicted target genes; the data obtained from biochip microarray was confirmed by real time RT-PCR examination.Results： The microarray revealed that EGCG treated Hep G2 cell expressed 27 differential lipid metabolism genes and 11 of them involved in cholesterol metabolism. In addition, there 285 lncR NA expressions were up- or down-regulated. Bioinformatics technology indicated that the predicted target genes for lipid metabolism might be cis- or trans-regulated by lncR NA; the data from real-time RT-PCR was consistent with the data from biochip microarray. Conclusion： Tea polyphenols improves lipid metabolism and lnc RNA might be involved in the regulation of lipid metabolism related gene.
出处
《中国循环杂志》
CSCD
北大核心
2014年第12期1039-1043,共5页
Chinese Circulation Journal
基金
国家自然科学基金(81241007)
