玉米花粉管通道法转化脱水素BDN1基因

Introduction of Dehydrin Gene(BDN1) into Maize by Pollen Tube Pathway

通过花粉管通道法将脱水素基因BDN1转入玉米自交系合344中,并对转化后代进行PCR和RT-PCR分子检测以及耐盐性功能鉴定,筛选耐盐性较高的转基因玉米新种质。结果表明,实验共获得88株T0代除草剂抗性植株,其中31株PCR检测呈阳性,14株RT-PCR检测呈阳性,对T4代转基因株系苗期进行300 mmol/L Na Cl溶液的盐胁迫处理,2个转基因株系耐盐性比对照提高两个级别。

Dehydrin gene BDNI was introduced into maize inbred line He344 by pollen tube pathway. Eighty- eight TO maize tranformed plants resisted to herbicide, among which 31 plants showed positive PCR reaction and 14 plants showed positive RT-PCR reaction. Eight transgenic maize lines with BDN1 gene were identified salt-toler- ance by observing the seedling appearance under 300 mmol/L NaC1 stress. Two transgenic maize lines have im- proved their salt tolerance ability by two-level compared to aeeeptors.