茯苓免疫调节蛋白-1抗体的制备及分析

Preparation and analysis of antibody against Poria cocos immunomodulatory protein-1

目的制备重组茯苓免疫调节蛋白-1(WCFIP1)及其抗体。方法合成WCFIP1基因、构建表达载体并转化大肠杆菌,表达并纯化重组蛋白,免疫小鼠、制备抗血清、纯化抗体并验证。结果纯化得到重组WCFIP1蛋白并制备特异性抗体。结论实现了重组WCFIP1蛋白的表达,纯化并制备了特异性较好的抗体。

Objective To prepare the recombinant Poria cocos immunomodulatory protein-1（WCFIP1） and its antibody. Methods The cDNA of WCFIP1 was synthesized, cloned into plasmid and transformed into Escherichia coli expression strain. The recombinant protein of WCFIP1 was expressed, purified, and injected into mice to prepare antiserum. The antibody against WCFIP1 was purified by portein-G column and the specificity was determined by Western blotting. Results The recombinant protein of WCFIP1 and the specific antibody were prepared. Conclusion The recombinant protein of WCFIP1 is expressed and purified from E. coli, and the specifity of the prepared antibody is determined.