狭基线纹香茶菜水溶性成分及其抗肿瘤活性研究

Water-soluble constituents from Isodon lophanthoides var. gerardianus and their antitumor activities

目的 研究狭基线纹香茶菜Isodon lophanthoides var.gerardianus的水溶性成分及其抗肿瘤活性.方法 采用大孔吸附树脂D-101、ODS、Sephdex LH-20等色谱技术和重结晶进行分离纯化,通过ESI-MS、1H-NMR、13C-NMR等现代波谱技术进行结构鉴定；以HepG2为供试细胞株,采用MTT法对化合物进行体外抗肿瘤活性研究.结果 从狭基线纹香茶菜水提液中分离得到12个水溶性化合物,分别鉴定为新西兰牡荆苷Ⅱ （1）、新西兰牡荆苷Ⅲ （2）、异夏佛塔苷（3）、夏佛塔苷（4）、牡荆苷（5）、芹菜素-6,8-二-Gα-L-吡喃阿拉伯糖苷（6）、芹菜素-7-O-葡萄糖醛酸苷（7）、芹菜素-6-C-β-L-吡喃阿拉伯糖-8-C-α-L-吡喃阿拉伯糖苷（8）、芹菜素-6-C-β-D-木糖-8-C-α-L-阿拉伯糖苷（9）、咖啡酸（10）、迷迭香酸（11）、芦丁（12）.结论 化合物1～9为首次从香茶菜属植物中分离得到；其中化合物1、6、11对HepG2细胞有较好的抑制作用.

Objective To investigate the water-soluble constituents oflsodon lophanthoides var. gerardianus. Methods Compouds were isolated and purified by D-101, Sephadex LH-20, and ODS column chromatographies. The chemical structures were elucidated on the basis of spectral data and physicochemical properties. Results Twelve compounds were isolated and identified as vitexin II （1）, vicenin III （2）, isoschat$Oside （3）, schaftoside （4）, vitexin （5）, 6, 8-di-C-α-L-arabinosylapigenin （6）, apigenin-7-O-glucuronide （7）, apigenin 6-C-β-L-arabin-osyl-8-C-α-L-arabinopyranoside （8）, apigenin 6-C-β-D-xylopyranosyl-8-C-α-L-arabinopyranoside （9）, caffeic acid （10）, rosmarinic （11）, and rutin （12）. Conclusion Compounds 1-9 are separated from 1. lophanthoides var. gerardianus for the first time, and constituents of the water-soluble parts ofL lophanthoides var. gerardianus are studied systematically for the first time. Comoounds 1, 6, and 11 exert anfiproliferative effects on HepG2 cells.