分子印迹电极法测定知柏地黄丸中盐酸小檗碱

Determination of berberine hydrochloride in Zhibai Dihuang Pills with ion selective electrode based on molecularly imprinted polymers

目的 建立一种知柏地黄丸中盐酸小檗碱（BH）定量测定的方法.方法 以BH为目标分子,甲基丙烯酸（MAA）为功能单体,乙二醇二甲基丙烯酸酯（EGDMA）为交联剂制备出BH分子印迹模板,以此模板为电活性物质,制备出BH玻璃涂膜离子选择性电极,采用工作曲线法测定了知柏地黄丸中BH的量.结果 当BH的浓度在1～100 μmol/L,与电动势（E）呈现良好的Nemst响应,斜率为55.48 mV/pC,回收率为94.5％～105.1％.结论 该方法测定快速、准确、灵敏,结果良好.

Objective To develop a method for the determination ofberberine hydrochloride （BH） in Zhibai Dihuang Pills. Methods The BH ion selective electrode was fabricated with a molecularly imprinted polymer （MIP）. The process is based on the synthesis of polymers with BH as template, methacrylic acid （MAA） as functional monomer, and ethylene glycol dimethacrylate （EGDMA） as a crosslinker. An ion selective electrode was prepared with this MIP as electric active substance. The content of BH was calculated with working curve. Results The electromotive force （E） exhibited a good Nemst response to BH concentration over a range of 1-100 μmol/L with a slop of 55.48 mV per decade, and recovery rate was 94.5%--05.1%. Conclusion The method is rapid, precise, and accurate, and the result is satisfaction.