离体猪心脏的保存及功能评价

Preservation and cardiac function evaluation of isolated heart from pig in vitro

目的:探索一种新型供体心脏体外保存及功能评价的方法。方法:将血型一致的12头瑞典健康家猪作为实验对象,随机分为心脏供体组和血液供体组两组,每组6头。用体外循环机以不同流量(0.76、1.00、2.00、3.00、4.00 L/min)在37℃条件下灌注离体心脏,监测动静脉血气和离体心脏血压、心率、心肌耗氧量及氧摄取率。结果:不同灌注流量下,离体心脏心肌耗氧量、心率、收缩压、平均压、左室收缩压差异均有统计学意义(F=23.500,331.010,77.701,10.101和89.301,P均<0.001)。灌注流量较高,离体心脏上述各指标也较高(F=0.460,P>0.05)。不同灌注流量组心肌氧摄取率差异无统计学意义(F=0.426,P=0.788)。结论:该模型可使离体心脏心功能保持良好并得到较好的保存。

Aim：To explore a new pig isolated heart preservation and heart function evaluation method .Methods：A total of 12 Swedish health home pigs were divided into two groups according to blood type , the blood donor group and isolated heart donor group, and 6 in each group.The cardiopulmonary bypass machine were used to perfuse isolated hearts at different perfusion flow（0.76,1.00,2.00,3.00, and 4.00 L/min） under normal temperature （37 ℃）.The myocardial oxygen con-sumption, heart rate, blood pressure and hemodynamic index of isolated hearts were monitored .Results：There were signifi-cant differences in myocardial oxygen consumption , heart rate, systolic blood pressure , average pressure , left ventricular sys-tolic blood pressure of isolated hearts with different perfusion flow （F=23.500,331.010,77.701,10.101 and 89.301,P〈0. 001）.The perfusion flow was higher , the indexes mentioned above were higher in isolated heart （P〈0.05）.There was no sta-tistically significant difference in myocardial oxygen uptake rate among the 5 groups（F=0.426,P=0.788）.Conclusion：The method for pig isolated heart preservation and accurate evaluation of cardiac function is established .