杨树PtEBP1基因转化拟南芥研究

Genetic transformation of the Pt EBP1 cloned from Populus into Arabidopsis thaliana

EBP1是一个可能在植物器官大小发育调控中发挥重要作用的基因,但其在木本植物如杨树中的功能仍未知。在前期获得杨树PtEBP1基因并构建相应表达载体pBI121-PtEBP1基础上,利用花序侵染法遗传转化拟南芥,进而通过观察转基因拟南芥的性状变化,对PtEBP1在器官发育过程中的功能进行初步探究。结果表明,杨树PtEBP1基因已成功转化拟南芥,转基因拟南芥表现为植株茎增粗、叶片明显增大且种子数量增多等显著有别于野生型的性状特征。该研究结果初步证实PtEBP1在植物器官发育,特别是器官大小发育中的重要作用。

EBP1 is one of the important genes that may regulate the plant organ development,however,the function of EBP1 remains unknown in woody plants,for example,in Populus. Based on the cloning of Pt EBP1 cloned from Populus and the construction of the expression vector p BI121-Pt EBP1,the p BI121-Pt EBP1 was transformed into Arabidopsis thaliana via floral-dip method. Then the function of Pt EBP1 in organ development was preliminary explored by observing the morphological characters of transgenic A. thaliana. The results showed that Pt EBP1 had been successfully transformed into A. thaliana,the transgenic A. thaliana exhibited many different characters from the wild type,such as the thicker stem,the bigger leaves and more seeds. This study preliminary confirmed that Pt EBP1 played important role in plant organ development,especially in the development of organ size.