P27^(KIP1)、P21^(WAF1)、Bax在胃肠道间质瘤中的表达及临床意义

Expression and significance of P27^(KIP1), P21^(WAF1), Bax in gastrointestinal stromal tumors

目的探讨P27KIP1、P21WAF1和Bax在胃肠道间质瘤中的表达及其与临床生物学行为的相关性。方法采用免疫组化法检测P27KIP1、P21WAF1和Bax在胃肠道间质瘤中的阳性表达情况,并在良性组、潜在恶性组、恶性病例组之间进行比较。结果 55例胃肠道间质瘤中,P27KIP1、P21WAF1和Bax的阳性表达率分别为36.3%、54.5%、60%。P21WAF1、Bax在良性组的表达分别为21.4%、28.6%,而在潜在恶性组、恶性组明显增高。P27KIP1在良性组的表达率为64.3%,明显高于潜在恶性组、恶性组(21.4%、29.6%)。P27KIP1、P21WAF1和Bax三者在潜在恶性组、恶性组的表达与良性组比较差异有统计学意义,而潜在恶性组与恶性组之间差异无统计学意义。P27KIP1、P21WAF1和Bax的表达与胃肠道间质瘤患者的性别、年龄、部位、肿瘤大小、有无坏死、组织学分型无关,而与其恶性潜能分级相关。结论 P27KIP1的低表达、P21WAF1和Bax的高表达与胃肠道间质瘤临床恶性潜能密切相关。

Objective To investigate the correlation between biological behavior and expression of P27^KIP1,P21^WAF1 and Bax in gastrointestinal stromal tumors. Methods Immunohistochemical method was used to detect P27^KIP1,P21^WAF1 and Bax expression in 55 cases of GIST, which were compared among benign GIST, potential malig-nant GIST, and malignant GIST. Results In 55 cases of GIST, the positive expression rates of P27^KIP1,P21^WAF1 and Bax were 36.3%, 54.5%, 60%. The expression rate of P21WAF1 and Bax were 21.4%, 28.6% in benign GIST, which were significantly increased in potential malignant GIST and malignant GIST. The expression rate of P27KIP1 in benign GIST （64.3%） was significantly higher than that in potential malignant GIST （21.4%） and malignant GIST （29.6%）. The expression rate of P27^KIP1,P21^WAF1 and Bax showed statistically significant difference between benign GIST and po-tential malignant GIST, malignant GIST, but showed no statistically significant difference between potential malignant GIST and malignant GIST. The expressions of P27^KIP1,P21^WAF1 and Bax showed no correlation with age, sex, location, size, necrosis and histological scheme, but they were related with the malignant progression. Conclusion The low ex-pression of P27KIP1, the high expression of P21WAF1 and Bax are closely related to clinical malignant potential of GIST.