玉壶汤对db/db小鼠糖尿病的药效学研究

Pharmacodynamics Study of Yuhu Decoction on Diabetes in db/db Mice

目的观察玉壶汤对db/db小鼠的降糖调脂作用,并比较党参代替人参后新方与原方的药效。方法取12周龄自发性2型糖尿病db/db小鼠,将血糖值大于16.7 mmol·L-1的小鼠按血糖水平分层随机分为模型组、玉壶汤组、玉壶汤新方组、消渴丸组,另设野生型雄性db/db小鼠为正常对照组。给药8周后,以生化仪检测小鼠血糖、总胆固醇(CHOL)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C);酶标仪检测小鼠血清超氧化物歧化酶(SOD)、丙二醛(MDA)、胰岛素、白介素-6(IL-6)含量。结果与模型组比较,玉壶汤组小鼠血清中血糖、CHOL、TG、MDA和IL-6含量显著降低,SOD活力、胰岛素水平显著升高(P<0.05,P<0.01);玉壶汤新方组小鼠血清中血糖、CHOL、TG和IL-6含量显著降低,SOD活力、胰岛素水平显著升高(P<0.05,P<0.01)。结论玉壶汤对2型糖尿病有较好的降糖调脂作用,以党参代替人参入方后的新方与原方的药效相当。

Objective To study the pharmacodynamics effects of Yuhu Decoction（YD）on lowering the level of blood glucose and lipid in db/db mice, and to compare the pharmacodynamic actions of the original decoction with the decoction with Radix Ginseng replaced by Radix Codonopsitis. Methods Spontaneous type 2 diabetes db/db mice aged12 weeks with blood glucose level over16.7 mmol·L-1were randomized into model group, YD group, modified YD group（YD with Radix Ginseng replaced by Radix Codonopsitis）,and Xiaoke Pills group according to the blood glucose level. Wild type male db/db mice served as the normal control. After medication with the corresponding drug for 8weeks,blood glucose and total cholesterol（TC）,triglyceride（TG）,high-density lipoprotein cholesterol（HDL-C）and low-density lipoprotein cholesterol（LDL-C） were detected by biochemical analyzer, Serum levels of superoxide dismutase（SOD）, malondialdehyde（MDA）, insulin and interleukin 6（IL-6） were detected by microplate reader according to kit method. Results Compared with the model group,the levels of glucose,TC,TG,MDA and IL-6 in blood were decreased, and SOD activities and insulin level were increased in YD group（P0.05,P〈0.01）; the observation indexes except the MDA showed the same changes in modified YD group（P0.05,P0.01）. Conclusion Y uhu Decoction has the effects of lowering the level of blood glucose and lipid in type 2 diabetes db/db mice,and the effect keeps steady when Radix Ginseng in the decoction is replaced by Radix Codonopsitis.