宫颈鳞癌组织中核因子κB蛋白的表达及其临床意义

Expression of Nuclear Factor Kappa B Protein in Cervical Squamous Cell Carcinoma Tissues and Its Clinical Significances

目的探讨宫颈鳞癌及宫颈上皮内瘤变(CIN)组织中核因子κB(NF-κB)的表达及其与宫颈癌临床病理因素的关系。方法采用免疫组化SP法检测正常宫颈20例(对照组),CIN 64例(CINⅠ26例,CINⅡ/Ⅲ38例),宫颈鳞癌45例(宫颈鳞癌组)宫颈组织中NF-κB p65蛋白的表达情况。结果对照组、CINⅠ组、CINⅡ/Ⅲ组、宫颈鳞癌组NF-κB p65蛋白阳性表达率分别为15.00%(3/20)、34.62%(9/26)、47.37%(18/38)、66.67%(30/45);CINⅡ/Ⅲ组、宫颈鳞癌组NF-κB p65蛋白阳性表达率均明显高于对照组(P<0.05);CINⅠ组NF-κBp65蛋白阳性表达率与对照组比较,差异无统计学意义(P>0.05)。宫颈鳞癌患者NF-κB p65蛋白阳性表达率与临床分期及淋巴结转移有关(P<0.05),但与组织学分级、肿瘤直径、年龄等临床病理因素均无明显关系(P>0.05)。结论宫颈鳞癌组织中NF-κB p65蛋白异常高表达,其阳性表达率与临床分期及淋巴结转移有关。

Objective To explore the relationship between the expression of nuclear factor kappa B（ NF-κB）and the clinicopathological characters in cervical squamous cell carcinoma and cervical intraepithelial neoplasia（ CIN）tissues. Methods Immunohistochemical SP method was used to detect the expression of NF-κBp65 protein in 20 cases of normal cervical tissue（ control group）,64 cases of CIN（ 26 of CINⅠ,38 of CINⅡ/Ⅲ） and 45 cases of cervical squamous cell carcinoma（ cervical squamous cell carcinoma group）. Results The positive expression rates of NF-κBp65protein in the control group,CINⅠ group,CINⅡ/Ⅲ group and cervical squamous cell carcinoma group were 15. 00%（ 3 /20）,34. 62%（ 9 /26）,47. 37%（ 18 /38） and 66. 67%（ 30 /45）,respectively. The positive expression rates of NF-κBp65 protein in CINⅡ/Ⅲ and cervical squamous cell carcinoma groups were significantly higher than that in the control group（ P〈0. 05）.But there was no significant difference in the positive expression rate of NF-κBp65 protein between CINⅠ group and control group（ P〉0. 05）. The positive expression rate of NF-κBp65 protein correlated with clinical stages and lymph node metastasis（ P〈0. 05）,but did not significantly correlate with the other clinicopathological characters including histological grade,tumor size and gender（ P〉0. 05）. Conclusion The NF-κBp65 protein is overexpressed in cervical squamous cell carcinoma tissues. Its positive expression rate correlates with clinical stages and lymph node metastasis（ P〉0. 05）.