南宁市壮族和汉族女性MTHFR和MTRR基因单核苷酸多态性比较

A Comparison of Single Nucleotide Polymorphisms of MTHFR and MTRR in Women Between Chinese Zhuang and Han Population in Nanning City

目的分析南宁市壮族和汉族女性5,10-亚甲基四氢叶酸还原酶(MTHFR)C677T、A1298C及甲硫氨酸合成酶还原酶(MTRR)A66G单核苷酸多态性的分布特征。方法以南宁市948例孕检女性为研究对象,其中壮族455例,汉族493例,采集口腔黏膜上皮细胞,提取5,10-DNA,通过荧光定量PCR方法进行MTHFR和MTRR基因多态性检测。统计分析本地区壮族和汉族女性基因多态性的分布特征。结果壮族女性MTHFR C677T位点TT基因型比例为5.1%,低于汉族女性(7.7%);MTHFR A1298C位点CC基因型比例为9.2%,高于汉族女性(5.5%);MTRR A66G位点GG基因型比例为9.7%,高于汉族女性(5.5%)。MTHFR C677T和MTHFR A1298C两位点多态性在壮族和汉族女性间的分布差异无统计学意义(P>0.05),而MTRR A66G位点多态性在两民族女性间的分布差异有统计学意义(P<0.05)。结论南宁市壮族和汉族女性MTRR基因多态性分布情况不同,具有民族特异性。

Objective To explore the distribution frequencies of single nucleotide polymorphisms of methylenetetrahydrofolate reductase（ MTHFR） C677 T,A1298C and methionine synthase reductase（ MTRR） A66 G among Zhuang and Han women in Nanning City. Methods A total of 948 women in Nanning City were recruited in the study,455 cases of Zhuang nationality and 493 cases of Han nationality. Their oral epithelial cell samples were collected,followed by the extraction of genome DNA,in order to detect gene polymorphisms of MTHFR and MTRR by using fluorescence quantitative PCR. The distribution frequencies of gene polymorphisms of Zhuang and Han women were analyzed. Results The TT genotype ratio of MTHFR C677 T of Zhuang women in Nanning City was 5. 1%,which was lower than that（ 7. 7%） of Han women. The CC genotype ratio of MTHFR A1298 C of Zhuang women in Nanning City was 9. 2%,which was higher than that（ 5. 5%） of Han women. The GG genotype ratio of MTRR A66 G of Zhuang women in Nanning City was 9. 7%,which was higher than that（ 5. 5%） of Han women. The polymorphism distribution of MTHFR C677 T and MTHFR A1298 C showed no significant difference between Zhuang and Han women（ P〉0. 05）,but the polymorphism distribution of MTRR A66 G showed the significant difference（ P〈0. 05）. Conclusion The polymorphism distribution of MTRR of Zhuang women is different from that of Han women in Nanning City,with ethnologic specificity.