苦参碱通过线粒体通路诱导HepG2细胞凋亡的机制研究

Matrine Induces Apoptosis of Hep G2 Cells via Mitochondrial Apoptotic Pathway

目的探讨苦参碱通过线粒体通路诱导Hep G2发生凋亡的机制。方法分别采用MTT法、PI染色法、流式细胞术检测苦参碱对肝癌Hep G2细胞的增殖抑制、周期调控、诱导凋亡作用;采用Western Blot检测苦参碱诱导肝癌Hep G2细胞抑凋亡蛋白Bid、Bcl-2的表达量。结果苦参碱可抑制Hep G2细胞的增殖作用和诱导凋亡,呈时间和剂量依赖性,并可将Hep G2细胞周期阻滞在G0/G1期。苦参碱可引起线粒体膜电位崩溃,同时诱导Bid、Bcl-2表达下调,Bax表达上调。结论苦参碱通过调节细胞内Bid、Bcl-2和Bax蛋白的表达,经线粒体信号转导途径诱导人肝癌细胞系Hep G2发生凋亡。

Objective To explore the mechanism of matrine inducing apoptosis of Hep G2 cells via mitochondrial apoptotic pathway. Methods MTT assay,PI staining,flow cytometry were used to evaluate the anti-proliferation,cell cycle regulation,and inducing apoptosis effects of matrine on Hep G2 cells. Western Blot assay was used to detect the expression levels of anti-apoptotic protein Bid and Bcl-2 in Hep G2 cells. Results Matrine could inhibit the proliferation and induce the apoptosis of Hep G2 cells in a time- and dose-dependent manner,and the cell cycle of Hep G2 was arrested in G0 / G1 phase. Apoptosis of Hep G2 cells induced by matrine was associated with collapse of mitochondrial membrane potential,which made the Bid and Bcl-2 expression levels down-regulated but the Bax expression level up-regulated. Conclusion Matrine induces the apoptosis of human hepatoma cell line Hep G2 via the mitochondrial apoptotic pathway by regulating the expression levels of Bid,Bcl-2 and Bax proteins.