一点红化学成分分离及其原儿茶酸含量测定

Isolation of chemical constituents of Emilia sonchifolia and determination of protocatechuic acid

为开发利用一点红植物的药用价值，以水提醇沉法提取一点红药材，采用极性分段结合半制备高效液相色谱法分离提取一点红水提物乙酸乙酯萃取部位，并根据理化性质和波谱数据确定其化合物结构，结果从一点红全草药材中分离得到5个单体化合物，经鉴定分别为对羟基苯乙酸（1）、对羟基苯甲酸（2）、对羟基肉桂酸（3）、原儿茶酸（4）、丁香酸（5），除化合物2外的4个化合物均为首次从该植物中分离得到；并采用外标法测定一点红中原儿茶酸的含量，其标准曲线在10~100μg范围内线性关系良好（R2=0.9995），该方法准确度高、重复性好，是一种快速有效的检测一点红中原儿茶酸含量的方法。

For the development and utilization of the medicinal worth of Emilia sonchifolia plants, the chemical constituents of Emilia sonchifolia were extracted with water extraction and alcohol pre-cipitation methods. Combined with the use of sub-polar semi-preparative high performance liquid chromatography to isolation the parts of ethyl acetate extract of Emilia sonchifolia aqueous extract and determine the structure of a compound based on their physicochemical properties and spectral data. Five compounds were isolated from the Emilia sonchifolia, which were identified as：4-hydroxyphe-nylacetic acid （1）, 4-hydroxybenzoic acid （2）, 4-hydroxycinnamic acid （3）, protocatechuic acid （4）, and syringic acid （5） . All compounds except compound 2 were isolated from this plant for the first time. The determination of protocatechuic acid in Emilia sonchifolia was performed using ex-ternal standard method. The standard curve of protocatechuic acid showed a good linearity in the range of 10~100 μg of concentration with R2=0. 999 5. The method developed in this study was rapid, accurate and easy to be used, therefore it would be good for determination of protocatechuic acid content in Emilia sonchifolia.