摘要
采用HPLC法测定磷酸西格列汀的含量.方法：采用C18（4.6 mm×215;250 mm,5μm）色谱柱,以0.01 mol/L磷酸氢二钠溶液（用磷酸调节pH至6.8）∶乙腈=60∶40为流动相,流速为1.0 mL/min,检测波长为220 nm,柱温为30℃.结果：磷酸西格列汀的线性范围为0.2300～0.3942mg/mL;结论：本法简便、准确、适合于磷酸西格列汀含量测定.
To establish an HPLC method for quantitative determination of Sitagliptin phosphate. Methods： The analytical column was C18 （4.6 mm×250 mm, 5 μm） cohmm. The mobile phase was 0.01 mol/L disodium hydrogen phosphate aqueous solution （phosphoric acid to adjust to pH 6.8） and acetonitrile （60 ： 40） at a flow rate of 1.0 mL/min. The wavelength of UV detector was 220 nm and column temperature was 30℃. Results： The linear ranges of Sitagliptin phosphate were 0.2300-0.3942 mg/ml Conclusion： The method is simple, accurate and suitable for the content determination of Sitagliptin phosphate,
出处
《广东化工》
CAS
2014年第23期204-204,共1页
Guangdong Chemical Industry
关键词
磷酸西格列汀
HPLC法
含量测定
sitagliptin phosphate
HPLC method
content determination
