3个重要酶的多克隆抗体的制备条件优化

Preparation Optimization of Polyclonal Antibodies Against Three Important Enzymes

目的制备优质的抗几丁质酶、α-甘露糖苷酶、谷胱甘肽转硫酶的多克隆抗体。方法利用纯化的不同免疫剂量的3种酶分别免疫不同兔龄的新西兰兔。结果使用0.3mg纯化蛋白、免疫6月龄的新西兰兔所制备出的抗体效价高、特异性好。结论这一结果为今后规模化制备相关多克隆抗体奠定了坚实的实验基础。

OBJECTIVE To prepare high-quanlity polyclonal antibody against chitinase,α-mannosidase and GST. METHODS Corresponding purified proteins with different doses were used to immunize the New Zealand rabbits of different ages. RESULTS When using 0. 3mg·L- 1purified proteins to immunize six-month-old New Zealand rabbit,the polyclonal antibodies possessed higher titer and specificity. CONCLUSION Our results establish a dependable basis for the large-scale production of the related polyclonal antibodies.