HPLC法同时测定蚕豆花中槲皮素和山奈酚的含量

The Simultaneous Content Determination of Quercetin and Kamepferol in Broadbean Flower by HPLC

目的建立HPLC法测定蚕豆花中的槲皮素和山奈酚含量的方法。方法测定槲皮素和山奈酚的色谱条件为色谱柱Inert Sustain C18（250mm×4.6mm,5μm）;以乙腈（A）∶0.4%磷酸水溶液（B）为流动相进行梯度洗脱,流速为0.8m L·min^-1;检测波长为365nm;柱温为30℃。结果槲皮素进样量在0.034~0.272μg范围内（r=0.9999）,山奈酚进样量在0.040~0.317μg范围内（r=0.9998）线性关系良好,平均回收率槲皮素为99.20%（RSD%=0.81,n=6）,山奈酚为98.39%（RSD%=1.49,n=6）。结论本法操作简便快捷、稳定性高、重现性好,为蚕豆花药材的质量评价提供依据。

OBJECTIVE To establish a analytical method on content determination of queretin and kaempferol in broadbean flower by HPLC. METHODS The chromatographic conditions were： column： Inert Sustain C18（250mm× 4. 6mm,5μm）; mobile phase： acetonitrile（A）-0. 4% phosphoric acid solution（B）（gradient elution）,flow rate at 0. 8m L· min^- 1,and detection wavelength at 365 nm,column temperature was 30℃. RESULTS The injection volume of quercetin was at the range of 0. 034 ~ 0. 272μg（r = 0. 9999）. The injection volume of kamepferol showed a good linear relationship at the range of 0. 040 ~ 0. 317μg（r = 0. 9998）,The average recovery was 99. 20% quercetin for（RSD% = 0. 81,n = 6） and 98. 39% for kamepferol（RSD% = 1. 49,n = 6）. CONCLUSION The method is simple and fast,which has high stability and good reproducibility and provide a basis for quality evaluating for broadbean flower as medicinal materials.