盐酸多塞平异构体的非水毛细管电泳分离

Determination of doxepin hydrochloride isomers by nonaqueous capillary electrophoresis

目的 用非水毛细管电泳法分离盐酸多塞平异构体。方法 采用未涂层石英毛细管柱 (50 μm× 47cm ,有效分离长度为 40cm) ,以用 2 0 0mmol·L- 1 醋酸铵的甲醇 乙腈 (95∶5)溶液 (含 3 .0 %冰醋酸 )为运行缓冲液 ,压力进样 ,时间 4s,运行电压为 2 5kV ,检测波长为 2 54nm。结果 方法分离效率高 ,精密度和回收率较好 ,所选浓度范围内线性关系良好 ,r值为 0 .9998。结论 方法简单可靠 ,分析时间短 。

OBJECTIVE: To establish a method based on nonaqueous capillary electrophoresis for the separation of Z-, E- isomers of doxepine hydrochloride. METHODS: The separation was performed on a fused-silica capillary of 50 μm × 47 cm (40 cm of effective length), and the running buffer was methanol-acetonitrile (95:5) containing 200 mmol&middotL-1 ammonium acetate and 3.0% glacial acetic acid. Samples were injected by pressure mode. The applied voltage was 25 kV and the detection wavelength was set at 254 nm. RESULTS: The method was validated in terms of reproducibility, linearity, recovery, limits of quantitation and detection. CONCLUSION: The method appeared to be simple and fast for the separation of Z-, E-isomers of doxepine hydrochloride.