紫草素提取工艺优化的研究

Research on the extracting process of alkannin from radix arnebiae seu lithospermi

目的探讨紫草素最佳提取工艺条件。方法选取紫草1kg，采用正交实验方法，选用乙醇、石蜡作为复合溶媒，用回流的方法提取萘醌色素类物质，观察其工艺的合理性。结果最佳条件组合为：A。B：C，D。，即A取20倍于药材质量的乙醇（如取紫草1kg，则取乙醇20kg）；B石蜡占乙醇用量的比例为5％（如取紫草1kg，取石蜡1kg）；c回流时间为2h；D回流次数为3次。结论取10倍于药材质量的乙醇；石蜡占乙醇用量的比例为5％9回流时间为1h，回流次数为2次，为更切合生产实际、节能、便捷的工艺条件。

Objective Optimizing the preparation technology of Red buttock ointment,by exploring the best extraction conditions of the alkannin, radix arnebiae seu lithospermi polysaccharides and so on. Methods Selecting radix amebiac seu lithospermi 1kg and using orthogonal experiment to explore the best extraction conditions. Naphtho- quinone pigment chemicals were extracted by reflux extraction method;ethanol and paraffin wax were chosen as the composite solvent. Radix arnebiae seu lithospermi polysaecharide was extracted by water decocting, which to observe the rationality of process. Results The best extraction condition is A3B2CIDl , including section A： ethanol was 20 times the quality of medicinal materials （select radix arnebiae seu lithospermi lkg and ethanol 20kg）, section B： the amount of paraffin was 5% of ethanol（select radix arnebiae seu lithnspermi lkg and paraffin 1 kg） ,section C： Boiling water was 20 times the quality of medicinal materials, section D： the boiling time was 2h. Conclusion The more practical production process conditions：Ethanol was 10 times the quality of medicinal materials, and the amount of paraffin was 5% of ethanol. Boiling water was 10 times the quality of medicinal materials, and the boiling time was 1 h. This method is economic, simple, reasonable, stable, reproducible and easy to operation. Red buttock ointment obtained by this method possesses of excellent quality, good performance and superior effect.