小容量肺灌洗治疗对矽肺合并COPD患者血清转化生长因子-β1和肿瘤坏死因子-α的影响

Effect of BAL on transforming growth factor β1( TGF-β1) and tumor necrosis factor α( TNF-α)in serum of the patients with silicosis merging COPD

目的评价小容量肺灌洗对矽肺合并慢性阻塞性肺疾病(COPD)患者血清中转化生长因子-β1(TGF-β1)和肿瘤坏死因子-α(TNF-α)的影响,从免疫学角度为小容量肺灌洗治疗矽肺合并COPD提供依据。方法选取江苏省无锡市第八人民医院尘肺科2012年8月—2013年6月门诊及住院确诊的矽肺合并COPD患者,患者自愿加入并签署知情同意书,随机分为肺灌洗组和对照组,采用酶联免疫吸附试验(ELISA)检测矽肺合并COPD患者治疗前后血清中TGF-β1和TNF-α的含量。结果治疗1个月后与治疗前比较,肺灌洗组及对照组血清TGF-β1、TNF-α水平均有下降,差异有统计学意义(P<0.05),但治疗后肺灌洗组的2种细胞因子水平与对照组比较下降差异有统计学意义(P<0.05)。结论小容量肺灌洗治疗能降低矽肺合并COPD患者血清中TGF-β1及TNF-α的水平。

[ Objective] To evaluate the effect of Bronchoalveolar Lavage（BAL） on transforming growth factor-β1 （ TGF-β1 ） and tumor necrosis factor-α （ TNF-α ） in serum of the patients suffered from silicosis merging COPD, and provide a basis in immunology of BAL in silicosis merging COPD treatment. [ Methods] The patients of silicosis merging COPD in the outpatient department and inpatient department in The Eighth People＇s Hospital of Wuxi City from Aug 2012 to Jun 2013 were chosen for study. All the patients were voluntary in experiment and signed the informed consents. The patients were randomly divided into 2 groups, the BAL group and the control group. The amount of TGF-β1 and TNF-ct in serum of the patients were tested before and after the treatment by the method of ELISA. [Results] Compared with it before treatment; after one month treatment, the amount of TGF-β1 and TNF-α in both BAL group and control group declined, of which the difference showed significant difference（ P 〈0.05 ）. However, compared the amount of the two cell factors in BAL group with it in control group, the decline showed significant difference （P 〈0.05）. [Condusion]BAL can decrease the amount of TGF-β1 and TNF-α in serum of the patients with silicosis merging COPD.