N端色氨酸修饰增强hemokinin-1拮抗绿脓杆菌的活性

N-Terminal Tryptophan-Tag Increases Antibacterial Activity of Hemokinin-1 Against P. Aeruginosa

为了明确色氨酸修饰是否增强HK1拮抗PA的活性,设计并合成了HK1及分别在其N端或C端进行的4个色氨酸修饰的短肽,采用猪皮模型测定了HK1及其色氨酸修饰肽对PA侵染率的影响,利用PA小鼠模型测定了HK1及其色氨酸修饰肽对PA感染小鼠的存活率及小鼠器官形态、重量的影响。结果表明:HK1及色氨酸修饰HK1能够显著降低PA对猪皮的侵染能力。其中,HK1的N端色氨酸修饰肽使PA相对侵染率降至3.4%,比HK1及其C端色氨酸修饰肽拮抗PA活性更强;HK1及其色氨酸修饰肽可增强小鼠抗PA感染能力,增加PA感染小鼠的存活率,减轻PA对主要器官的损害,其中HK1的N端色氨酸修饰肽促进小鼠抗PA感染的活性最强。

To determine whether tryPtoPhan-tag enhancing the activity of HK1 against P. Aeruginosa, we designed and synthesized HK1 and W-tagged HK1 which has 4 tryPtoPhan resPectively on the C-terminal or N-terminal. The Pig skin model was adoPted to determine HK1 and W-tagged HK1 antago-nistic activity on against P. Aeruginosa . The PA mouse model was aPPlied to determination the surviv-al rate of the infected mice. Changes of morPhology and weight of the infected mice were also observed after being treated by HK1 or W-tagged HK1 . The results show that the HK1 or W-tagged HK1 signifi-cantly reduces the infection rate of P. Aeruginosa to the Pig skin invasion. N-terminal W-tagged HK1 has stronger activity to antagonism P. Aeruginosa than HK1 or C-terminal W-tagged HK1with the in-fection rate falling to 3. 4%. HK1 or W-tagged HK1 enhances the ability of mice to resist P. Aerugi-nosa infection,and increases the survival rate of mice,and reduces the damage to the major organs of mice. N-terminal W-tagged HK1 Promotes the strongest antagonistic activity to Protect mouse to avoid being damaged by P. Aeruginosa .