淋巴细胞白血病和淋巴瘤T细胞受体基因重排研究

Study of TCR gene rearrangement in acute lymphoblastic leukemia and lymphoma

目的:探讨淋巴细胞白血病及淋巴瘤的T淋巴细胞受体(TCR)Vβ亚家族分布特点。方法:用逆转录聚合酶链反应(RT-PCR)检测急性淋巴细胞白血病5例,慢性淋巴细胞白血病2例,弥漫大B淋巴瘤3例及外周T淋巴瘤3例患者的外周血或淋巴结T淋巴细胞TCR Vβ亚家族表达。对照组为健康人8例。结果:对照组健康8例外周血淋巴细胞表达全部24个Vβ亚家族。急性淋巴细胞白血病和慢性淋巴细胞白血病患者外周血淋巴细胞TCR Vβ亚家族均呈限制性表达,外周T淋巴瘤2例和弥漫大B淋巴瘤1例Vβ亚家族呈限制性表达,另外3例表达未受抑制。而淋巴瘤患者淋巴结标本Vβ亚家族全部呈限制性表达。结论:TCR基因重排对淋巴瘤和淋巴细胞白血病的诊断有辅助作用。

Objective：To investigate the distribution characteristics of T lymphocyte T cell receptor （TCR） Vβsub_family in acute lymphoblastic leukemia and lymphoma. Methods： The expression of TCR Vβ subfamilies in peripheral blood or lymph nodes was detected by reverse transcription polymerase chain reaction （RT-PCR） among acute lymphoblas_tic leukemia （5 cases） and lymphoma patients （2 cases of chronic lymphocytic leukemia, 3 cases of diffuse large B lym_phoma, 3 cases of peripheral T lymphoma）. Results： The expression of TCR Vβ subfamilies was restricted in peripheral blood among acute lymphoblastic leukemia, chronic lymphocytic leukemia and three cases of lymphoma patients. However, the other three cases were not inhibited. The expression of Vβ subfamilies in lymph nodes specimens was all restricted. Conclusion：Detecting TCR gene rearrangements plays a supplementary role in the diagnosis of lymphoma or lymphoblastic leukemia.