青杨脊虎天牛伴生真菌研究

A study on associated fungi from Xylotrchus rusticus L.

为了探索青杨脊虎天牛(Xylotrechus rusticus L.)伴生真菌群落组成及其对杨树的致病性,利用稀释平板涂布法进行伴生真菌的分离、培养,从青杨脊虎天牛(X.rusticus L.)各虫态、寄主及其蛀屑等9种材料中共分离出72个菌株,分属于19个菌属。青杨脊虎天牛蛀入寄主杨树后,可以引起寄主的树皮以及木质部坑道内真菌群落发生明显变化。与健康杨树相比,青杨脊虎天牛虫体及其蛀道内真菌组成、优势菌种类型都存在明显差异。研究结果证明,茄病镰刀菌(Fusarium solani(Mart.)Sacc.)是青杨脊虎天牛幼虫、蛹及其蛀害树皮表面存在的优势菌种,该优势菌培养液对杨树水培枝条具有致病性,且随着作用时间延长,致病性增强。

In order to explore Xylotrchus rusticus L. associated fugal community composition and pathogenicity,dilution plate method was used to isolate fungi from X. rusticus L. instar,host frass that nine kinds of materials,and there were72 strains which belonged to 19 genera. After X. rusticus bore into the host poplar,fungi could cause significant changes within the host community bark and xylem sap. Compared with healthy poplar,X. rusticus and fungi within the bore path composition and dominant strains types were significantly different. Our study showed that Fusarrium solani（ Mart.）Sacc was Xylotechus beetle larvae,paupae and termite damage its dominant bacteria presented on the surface of bark.The dominant bacteria culture medium for hydroponic poplar branches had pathogenic,and with prolonged duration of action which enhanced pathogencity.