多发性骨髓瘤细胞系中ANGPT1/2基因的表达

ANGPT1/2 gene expression in the cell lines of multiple myeloma

目的检测ANGPT1/2基因在多发性骨髓瘤细胞系中的表达。方法应用实时荧光定量(RQ)-PCR方法检测两种多发性骨髓瘤细胞株U266和P3X63Ag653(653)中ANGPT1/2的表达。结果以人脐静脉内皮细胞为对照,ANGPT1在U266和653中的表达升高,与人脐静脉内皮细胞相比差异无统计学意义。ANGPT2在U266中表达降低,与人脐静脉内皮细胞相比差异无统计学意义;而ANGPT2在653中明显高表达,与人脐静脉内皮细胞相比差异有统计学意义。结论应用RQ-PCR检测ANGPT1/2在2株多发性骨髓瘤细胞系中表达,为下一步在体内外研究ANGPT-TIE在多发性骨髓瘤中的作用机制奠定基础。

Objective To investigate the mRNA expression of ANGPT1/2 gene in multiple myeloma cell lines.Methods Expression level of ANGPT1/2 in 2 kinds of multiple myeloma cell lines were assessed with the RQ - PCR.Results Using human umbilical vein endothelial cells as the normal control, the expression of ANGPT1 in U266 and 653ceils were elevated. There was no significant difference among them. The expression of ANGVI＇2 in U266 cells was downregulated with no singnificant difference when compared with normal control ; but the expression of ANGPT2 in 653 cellswas significantly up - regulated. Conclusion Expression level of ANGPT1/2 in 2 kinds of multiple myeloma cell lineshave been detected with the RQ - PCR, which provides the theoretical basis for the research of pathogenesis of ANGPT -TIE in multiple myeloma in vivo and in vitro.