HBV携带者外周血中辅助性T淋巴细胞1/辅助性T淋巴细胞2相关细胞因子的研究

Study about Th1/Th2 related cytokines in peripheral blood of patients with chronic HBV carriers

目的:研究慢性HBV携带者外周血中辅助性T淋巴细胞1(Th1)/辅助性T淋巴细胞2(Th2)相关细胞因子白细胞介素2(IL-2)、白细胞介素4(IL-4)及γ干扰素(γ-IFN)随患者体内病毒含量变化时的变化及其意义。方法:通过荧光定量方法检测86名慢性HBV携带者体内的病毒载量,而后根据病毒载量值将患者分为HBV DNA阴性组、HBV DNA低载量组、HBV DNA高载量组;通过ELISA方法检测86名慢性HBV携带者及39名正常人血清中IL-2、IL-4及γ-IFN的含量。结果:各患者组外周血清中IL-2的含量比正常组增高,且HBV DNA低载量组、HBV DNA高载量组与正常组比较,差异有统计学意义(F=3.055,P=0.031);各患者组外周血清中IL-4的含量比正常组增高,HBV DNA高载量组与正常组比较,差异有统计学意义(F=2.486,P=0.064);各患者组外周血清中γ-IFN的含量与正常组相比,差异无统计学意义(F=0.063,P=0.976)。结论:慢性HBV携带者体内存在Th1/Th2平衡紊乱,且可能Th1存在免疫功能障碍是形成的机制之一。

Objective： To investigate the changes of Thl/Th2 related cytokines IL-2, IL-4 andγ-IFN in peripheral blood of patients with chronic HBV carriers. Methods： HBV DNA loads were detected by fluorescent quantitative PCR. in 86 chronic HBV carriers, then patients were divided into three groups： HBV-negative group, low HBV DNA loads group and high HBV DNA loads group. The levels of IL-2, IL-4 and γ-IFN in peripheral blood of 86 chronic HBV carriers and 39 normal controls were analyzed by ELISA. Results： The levels of IL-2 in chronic HBV carriers increased compared with normal controls group, low HBV DNA loads group and high HBV DNA loads group significantly increased compared with normal controls group , （ F = 3. 055, P = 0. 031 ） ; The levels of IL-4 in chronic HBV carriers increased compared with normal controls group, high HBV DNA loads group significantly increased compared with normal controls group （ F = 2. 486, P = 0. 064） ; The levels of γ-IFN were no significant differences among three groups （ F = 0. 063, P = 0. 976 ） . Conclusion ： There have dysfunction in T helper cell （Th1/Th2） of patients with chronic HBV carriers, and it may be one of the mechanisms of immune dysfunction in Thl cells of patients with chronic HBV carriers.