IL-10基因内含子区基因多态性研究

Genetic polymorphism of interleukin 10 gene intron region

目的比较研究白细胞介素10(IL-10)基因内含子区单核苷酸多态性(SNP)基因型及等位基因在不同地区、种族人群之间的分布情况。方法采用单碱基延伸PCR技术和DNA测序方法来检测广西人群IL-10基因内含子区rs3021094T/G多态性,并与人类基因组计划研究的4个人群(欧洲、非洲、中国北京、日本)以及台湾地区人群SNP分型数据进行比较。结果 rs3021094T/G多态性分布的频率在广西人群男性、女性之间差异无统计学意义(P>0.05)。与日本、中国北京、台湾地区人群比较分布差异无统计学意义(P>0.05);而与欧洲、非洲地区人群比较,分布差异有统计学意义(P<0.05)。结论 IL-10基因内含子区基因多态性在不同地区、种族人群之间的分布有差异。

Objective To investigate the frequencies of genotype and allele distribution of interleukin 10（IL-10）gene intron region single nucleotide polymorphism（SNP）in Guangxi population,and to analyze allelic and genotypic diversity of IL-10 gene among different regions and races. Methods The polymerase chain reaction-single base extension（PCR-SBE）and DNA sequencing were used to analyze the rs3021094T/G polymorphisms at the IL-10 gene intron region in Guangxi population.The diversity of genotypic and allelic rs3021094T/GT polymorphisms at the intron region of IL-10 in Guangxi population were compared with others（Hapmap-CEU,Hapmap-YRI,Hapmap-HCB,Hapmap-JPT）from the Human Genome Project group（Hapmap）data（http：//www.ncbi.nlm.nih.gov/projects/SNP/）as well as Taiwan of China. Results Compared the frequencies of rs3021094T/G polymorphisms between females and males in Guangxi population,there were no significant differences（P〉0.05）.Compared the frequencies of rs3021094T/G polymorphisms of Guangxi population with HapMap-HCB,HapMap-JPT and Taiwan of China,there were no significant differences（P〉0.05）.But compared the frequencies of rs3021094T/G polymorphisms of Guangxi population with HapMap-CEU,HapMap-YRI population,there were significant differences（P〉0.05）.Conclusion The frequencies of polymorphisms distribution of IL-10 gene at intron region were significant differences among different regions and ethnic groups.