徐州地区猫源弓形虫虫株的分离与鉴定

Isolation and identification of Toxoplasma gondii strains from cats in Xuzhou region

目的获得徐州地区猫源弓形虫虫株,了解徐州地区流浪猫弓形虫感染情况。方法捕捉徐州地区流浪猫,收集血清后,用ELISA试剂盒检测弓形虫特异性Ig G抗体;同时将猫的心、脑、舌用盐酸-胃蛋白酶溶液消化处理后接种小鼠,经连续传代分离弓形虫虫株,采用特异PCR法对所分离的虫株进行鉴定。结果共捕捉41只流浪猫。从猫体内共分离到弓形虫虫株11株,PCR均扩增出弓形虫特异性条带;ELISA结果表明41.5%的流浪猫均能检测到弓形虫抗体。结论徐州地区流浪猫弓形虫感染较为严重,有可能成为人和其它动物弓形虫病的重要传染源,应引起相关部门的重视。

Objective To isolate Toxoplasma gondii（T. gondii）strains from stray cats and explore their prevalence in Xuzhou City. Methods The sera of 41 stray cats were collected to detect the antibodies of T. gondii by using a commercial enzymelinked immunosorbent（ELISA）kit. The tissues including the heart,brain and tongue from these cats were digested by acid pepsin solution and inoculated to Kunming mice. These suspicious isolates were subsequently identified by a specific PCR method.Results A total of 11 strains were isolated from 41 stray cats,which were confirmed by the PCR results. Moreover,17 cats（41.5%）were found to be positive with the antibodies of T. gondii. Conclusion A high prevalence of T. gondii infection was found in Xuzhou City,which indicates that the stray cats infected with T. gondii would be an important infection source that may infect humans and other animals in this area.