沃尔巴克氏体与淡色库蚊溴氰菊酯抗性关系初步研究

Presumptive role of Wolbachia in deltamethrin resistance of Culex pipiens pallens

目的探讨沃尔巴克氏体与淡色库蚊溴氰菊酯抗性间的相关性。方法以PCR鉴定本实验室饲养的淡色库蚊体内的沃尔巴克氏体,采用实时定量PCR技术(q RT-PCR)测定并比较淡色库蚊溴氰菊酯抗性和敏感品系中沃尔巴克氏体表达差异。结果本实验室饲养的淡色库蚊体内含有沃尔巴克氏体。淡色库蚊溴氰菊酯抗性品系卵、一龄蚊、二龄蚊、三龄蚊、四龄蚊、雌蚊、雄蚊体内沃尔巴克氏体表达水平分别是敏感品系的18.42、3.69、4.43、3.96、6.31、1.55、3.76倍,差异均有统计学意义(P均<0.05),而抗性品系和敏感品系蛹中沃尔巴克氏体表达水平差异无统计学意义(P>0.05)。南京江心洲溴氰菊酯抗性雌蚊体内沃尔巴克氏体表达水平是敏感雌蚊的2.64倍。结论沃尔巴克氏体可能与淡色库蚊对溴氰菊酯产生抗性有关。

Objective To investigate the relationship between Wolbachia and deltamethrin resistance in Culex pipiens pallens. Methods PCR was used to detect Wolbachia in Culex pipiens pallens and q RT-PCR was performed to determine and compare the expression of Wolbachia between deltamethrin- resistant and- susceptible strains of Culex pipiens pallens. Re Results Wolbachia was detected in Culex pipiens pallens in the laboratory. The expression of Wolbachia was 18.42,3.69,4.43,3.96,6.31,1.55 and 3.76 folds higher in the deltamethrin-resistant strain than in susceptible strain in the egg,1st,2nd,3rd,4th stages, and male and female adults, but there was no statistical difference in the pupae stage. The expression of Wolbachia was 2.64 folds higher in deltamethrin-resistant females than in susceptible females which were caught in Jiangxinzhou of Nanjing. Conclusion Wolbachia is associated with deltamethrin resistance in Culex pipines pallens.