五加皮体外抗甲型流感病毒的作用

Effects of cortex acanthopanacis against influenza A virus activity in vitro

目的筛选出五加皮最佳的给药方式并找出五加皮水浸液体外抗流感病毒的最大无毒浓度。方法采用流感病毒H1N1鸡胚传代,用血凝实验测定病毒效价;病毒感染细胞后观察CPE,采用Karber公式计算病毒的TCID50,用100TCID50的病毒液感染MDCK细胞;五加皮的水浸液作用于未经病毒感染的MDCK细胞48h,采用MTT法检测找出药物对细胞的最大无毒浓度。分别采用治疗给药、预防给药、直接灭活3种方式作用于MDCK细胞,观察药物对病毒的作用,并计算药物的抗病毒有效率(ER)。结果 1病毒感染MDCK细胞浓度为:100TCID50=2.14×10-3;2药物作用于MDCK细胞的最大无毒浓度为1.78 mg/m L,其细胞存活率高达99.4%;33种给药方式中,五加皮水浸液治疗给药组没有作用(ER﹤0),预防给药组(ER=78.26%)及直接灭活病毒组(ER=27.78%)抗病毒均有效,其中预防给药组的抗病毒有效率最高,作用效果最好。结论五加皮在体外具有较好的抗甲型流感病毒的作用,且预防给药方式抗病毒效果最好。

Objective To screen the optimal administration way and the maximum avirulent concentration of Cortex Acanthopanacis（ CA） against influenza virus activity in vitro. Methods Influenza A virus H1N1- transfected chicken embryos were passaged and the H1N1 titer was detected through the hemagglutination experiment.The Karber formula was used to measure the virus TCID50 by CPE with 100 TCID50 infecting MDCK cells. MDCK cells were treated by the infusion of CA for 48 h and MTT assay was used to evaluate the maximum non- toxic concentration of cells. The effects of three methods including CA treatment,CA pretreatment and direct inactivation of virus on virus in MDCK cells were investigated and the anti- virus effective rate（ ER） was calculated.Results 1） The 100TCID50 was 2. 14 × 10^- 3; 2） The maximum non- toxic concentration in MDCK cells was 1. 78 mg / ml and the cell survival rate was 99. 4%; 3） Among three treatment methods,CA pretreatment and direct inactivation of virus had anti- virus effects,in which ER in CA pretreatment group（ ER = 78. 26%） was better than that in direct inactivation of virus group（ ER = 27. 78%）. However,CA treatment had no significant anti-virus effects. Conclusion CA has anti- virus effects on influenza A virus in vitro with the best anti- virus activities of CA pretreatment.