盐胁迫下MaAQP1转基因拟南芥幼苗的生长和生理响应

Growth and physiological response of MaAQP1-transgenic Arabidopsis thaliana seedlings to NaCl stress

为了探讨盐胁迫对香蕉水通道蛋白质基因(Ma AQP1)转基因拟南芥幼苗的生长及抗逆性生理指标的影响,以Ma AQP1转基因拟南芥为研究对象,研究不同盐(Na Cl)浓度(0 mmol/L、50 mmol/L、100 mmol/L、150mmol/L)处理下转基因拟南芥的表型及生理指标变化。结果显示:(1)经过盐胁迫后,野生型和转基因株系钾离子(K+)浓度降低,钠离子(Na+)浓度升高,而转基因株系K+和Na+浓度均明显低于野生型株系,同时,转基因株系具有较高的K+/Na+;(2)随着盐胁迫浓度的增加,野生型和转基因株系中电导率、丙二醛含量、脯氨酸含量及过氧化氢酶活性均逐渐升高,当盐胁迫浓度达到150 mmol/L时4个指标均达到高峰,其中,转基因株系中的电导率、脯氨酸含量及过氧化氢酶活性均比野生型的高,而丙二醛含量比野生型的低;(3)随着盐胁迫浓度的增加,野生型株系幼苗的根长缩短程度大于转基因株系,同时,转基因株系的根毛要远多于野生型。表明Ma AQP1能够提高转基因拟南芥的耐盐性。

The changes of seedling growth and physiological indexes of Ma AQP1-transgenic Arabidopsis thaliana under different concentrations of Na Cl stress at 0 mmol/L,50 mmol/L,100 mmol/L,and 150 mmol/L were detected.Under Na Cl stress,K＋concentration in wildtype and transgenic plants were reduced,while Na＋concentration were increased.K＋and Na＋concentrations in transgenic plants were significantly lower than those in the wild-type plants,however,K＋/Na＋ratio was higher in transgenic lines.With the increase of salt concentration,the ion leakage,malondialdehyde content,proline content and catlyse（CAT） activity in both transgenic lines and wild type were increased gradually,and reached the peak values at salt concentration of 150 mmol/L.The ion leakage,proline content and CAT activity in the transgenic lines were higher than those in the wild-type plants,while the MDA content was lower.With the increase of salt concentration,the root length in wild-type lines seedling was shortened more than that in transgenic lines whose root hairs were far more than wild type＇s.These results suggested that Ma AQP1 could improve salt tolerance of transgenic A.thaliana.