pyrG基因对西瓜噬酸菌致病性和组氨酸利用的影响

Effect of pyrG gene on pathogenicity and utilization of histidine in Acidovorax citrulli

为研究pyr G基因与西瓜噬酸菌(Acidovorax citrulli)致病相关性,利用同源重组的方法构建pyr G基因突变体(Δpyr G),对pyr G基因的功能进行分析。结果显示,pyr G基因影响病原菌的致病性、游动性、生物膜形成和定殖能力以及激发烟草过敏反应能力;pyr G基因影响his D基因的表达;此外,pyr G基因突变影响西瓜噬酸菌对组氨酸的利用。

To study the relationship between pyr G and Acidovorax citrulli pathogenicity,pyr G gene mutant Δpyr G was constructed by homologous recombination method,and the function of pyr G gene was analyzed.Δpyr G from wild-type strain Acxj L12 in pathogenicity swimming motility,biofilm formation,colonization ability and hypersensitive response to tobacco.qRT-PCR revealed that pyr G affected the transcription of his D gene.The pyr G gene mutation played a negative role in histidine utilization by Acidovorax citrulli.