解草啶浸种减轻丙草胺对水稻药害的机制

Mechanism of fenclorim reducing the phytotoxicity of pretilachlor to rice by seed soaking

为揭示解草啶浸种减轻丙草胺对水稻药害的机制,采用生物化学方法研究解草啶浸种对水稻的谷胱甘肽巯基转移酶(GST)活性、α-淀粉酶活性、叶绿素含量和蛋白质含量的影响。结果显示:单独施用30 mg/L解草啶浸种能提高水稻GST活性,但对α-淀粉酶活性、叶绿素含量和蛋白质含量没有影响。单独施用不同剂量(56.25g/hm2、112.50 g/hm2、225.00 g/hm2)丙草胺后,低剂量丙草胺对GST活性有促进作用,高剂量丙草胺对GST活性有抑制作用;不同剂量丙草胺对α-淀粉酶活性、叶绿素含量和蛋白质含量均有抑制作用。通过30 mg/L解草啶浸种再施用不同剂量丙草胺,解草啶均能减轻丙草胺对水稻GST活性、α-淀粉酶活性、叶绿素含量和蛋白质含量的抑制作用。

To reveal the mechanism of fenclorim reducing the phytotoxicity of pretilachlor to rice by seed soaking,biochemical tests were conducted to study the effects of fenclorim soaking on glutathione S-transferase（GST） activity,α-amylase activity,and contents of chlorophyll and protein of rice.The results showed that GST activity was enhanced by 30 mg/L fenclorim,however,α-amylase,contents of chlorophyll and protein were not affected.Low dose of pretilachlor could increase GST activity while high dose showed inhibition.All doses of pretilachlor exhibited negative effect on α-amylase activity,contents of chlorophyll and protein.The negative effect on GST activity,α-amylase activity,contents of chlorophyll and protein of rice could be reduced by seed soaking with 30 mg/L fenclorim.