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桑树MaSAUR2基因的克隆、序列特征及时间特异性表达 被引量：2

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摘要 SAUR是生长素早期响应基因,能够被生长素快速诱导表达,本研究从桑树绿枝扦插的插穗基部皮层中克隆得到1个桑树SAUR家族基因,命名为Ma SAUR2(Gen Bank登录号:KC852881)。该基因序列全长1 186 bp,ORF全长549 bp,编码182个氨基酸,蛋白质分子质量为20 700,无信号肽与跨膜区域,在第82 aa至138 aa之间有1个保守的SAUR-specific结构域。桑树绿枝插穗基部经吲哚-3-乙酸(IAA)诱导处理,插穗基部皮层Ma SAUR2基因的表达水平在5 min内显著上升;在绿枝扦插生根过程中,不定根长出皮层时Ma SAUR2基因表达量显著升高。推测Ma SAUR2参与了桑树扦插过程中不定根的形成。
作者 李小玉 张晓峰 杜伟 聂浩 唐壮 班月圆 杜小龙 程嘉翎
机构地区 江苏科技大学 中国农业科学院蚕业研究所
出处 《江苏农业科学》 北大核心 2014年第12期34-38,共5页 Jiangsu Agricultural Sciences
基金 现代农业产业技术体系专项(编号:CARS-22)
关键词 桑树 MaSAUR2基因 基因克隆 序列特征 实时定量PCR
分类号 S888.2 [农业科学—特种经济动物饲养] Q943.2 [生物学—植物学]
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参考文献25

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共引文献9

同被引文献29

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引证文献2

二级引证文献8

江苏农业科学
2014年 第12期

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