期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

枯草杆菌启动子P43及Pamy的转录活性 被引量：2

下载PDF
导出
摘要 利用报告基因β-半乳糖苷酶,分析了枯草杆菌启动子P43及Pamy的转录活性。结果表明,在基本培养基条件下,启动子P43在对数前期开始表现转录活性,随菌量增大活性迅速提高,稳定期逐步平稳;在富营养培养基条件下,启动子P43转录活性在对数前期与基本培养基条件下相似,在对数期转录活性增加速率则高于基本培养基条件下的增加速率;启动子P43转录活性在E.coli和B.subtilis体内基本一致。启动子Pamy在淀粉诱导下转录活性较高,在添加葡萄糖以及无诱导条件下酶活较低。
作者 张婷婷 马磊
机构地区 石河子大学生命科学学院
出处 《江苏农业科学》 北大核心 2014年第12期56-58,共3页 Jiangsu Agricultural Sciences
基金 国家自然科学基金(编号:31272416) 石河子大学高层次人才科研启动专项(编号:RCZX201137) 石河子大学优秀青年项目(编号:2012ZRKXYQ13) 石河子大学青年骨干教师培养计划
关键词 枯草杆菌 启动子 转录活性 基因表达
分类号 Q933 [生物学—微生物学]
  • 相关文献

参考文献11

  • 1徐友强,马翠卿,陶飞,许平.细菌启动子识别及应用研究进展[J].生物工程学报,2010,26(10):1393-1403. 被引量：8
  • 2Wang P Z,Doi R H. Overlapping promoters transcribed by Bacillus subtilis sigma 55 and sigma 37 RNA polymerase holoenzymes during growth and stationary phases[J]. Journal of Biological Chemistry,1984,259(13):8619-8625.
  • 3Blombach B, Eikmanns B J. Current knowledge on isobutanol production with Escherichia coli,Bacillus subtilis and Corynebacterium glutamicum[J]. Bioengineered,2011,2(6):346-350.
  • 4Lu Y P,Lin Q,Wang J,et al. Overexpression and characterization in Bacillus subtilis of a positionally nonspecific lipase from Proteus vulgaris[J]. Journal of Industrial Microbiology & Biotechnology,2010,37(9):919-925.
  • 5Harwood C R,Cutting S M. Molecular biological methods for Bacillus:vol. 707[M]. New York:Wiley,1990.
  • 6Ye R Q,Kim J H,Kim B G,et al. High evel secretory production of intact,biologically active staphylokinase from Bacillus subtilis[J]. Biotechnology and Bioengineering,1999,62(1):87-96.
  • 7Lin X,Wong S L,Miller E S,et al. Expression of the bacillus licheniformis PWD-1 keratinase gene in B. subtilis[J]. Journal of Industrial Microbiology and Biotechnology,1997,19(2):134-138.
  • 8Yang S,Huang H,Zhang R,et al. Expression and purification of extracellular penicillin G acylase in Bacillus subtilis[J]. Protein Expression and Purification,2001,21(1):60-64.
  • 9Stülke J,Hillen W. Regulation of Carbon catabolism in Bacillus species[J]. Annual Review of Microbiology,2000,54(1):849-880.
  • 10Braaz R,Wong S L,Jendrossek D. Production of PHA depolymerase A(PhaZ5)from Paucimonas lemoignei in Bacillus subtilis[J]. FEMS Microbiology Letters,2002,209(2):237-241.

二级参考文献64

  • 1Mitchel J, Zheng D, Busby S, et al. Identification and analysis of extended -10 promoters in Escherichia coli. Nucleic Acids Res, 2003, 31(16): 4689-4695.
  • 2Browning DF, Busby SJ. The regulation of bacterial transcription initiation. Nat Rev Microbiol, 2004, 2: 57-65.
  • 3Travers AA, Burgess RR. Cyclic re-use of the RNA polymerase sigma factor. Nature, 1969, 222: 537-540.
  • 4Gruber TM, Gross CA. Multiple sigma subunits and the partitioning of bacterial transcription space. Annu Rev Microbiol, 2003, 57: 441-466.
  • 5Murakami K, Masuda S, Campbell EA, et al. Structural basis of transcription initiation: an RNA polymerase holoenzyme/DNA complex. Science, 2002, 296: 1285-1290.
  • 6Merrick MJ. In a class of its own--the RNA polymerase sigma factor σ^54 (σN). Mol Microbiol, 1993, 10: 903-909.
  • 7Gardan R, Rapoport G, Debarbouille M. Role of the transcriptional activator RocR in the arginine-degradation pathway of Bacillus subtilis. Mol Microbiol, 1997, 24: 825-837.
  • 8Debarbouille M, Verstraete IM, Kunst F, et al. The Bacillus subtilis sigL gene encodes an equivalent of σ^54 from gram-negative bacteria. Proc Natl Acad Sci USA, 1991, 88: 9092-9096.
  • 9Buck M, Gallegos MT, Studholme D J, et al. The bacterial enhancer-dependent σ^54 (σ^N) transcription factor. J Bacteriol, 2000, 182(15): 4129-4136.
  • 10Wang L, Gralla JD. Multiple in vivo roles for the 212-region elements of sigma 54 promoters. J Bacteriol, 1998, 180: 5626-5631.

共引文献7

同被引文献24

引证文献2

二级引证文献2

江苏农业科学
2014年 第12期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部