吸入硫化氢对大鼠肺组织氧化作用的影响

Oxidative stress of H_2S inhalation in lung of rats

目的探讨吸入硫化氢(H2S)不同时间对大鼠肺组织的氧化作用的影响。方法 18只雄性SD大鼠随机分成对照组、3 h组、6 h组,每组6只。对照组无H2S吸入,3 h组、6 h组分别在吸入H2S 3 h、6 h后ELISA法检测支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中谷胱甘肽(glutathione,GSH)、γ-谷氨酰半胱氨酸合成酶(γ-glutamyl cysteine synthetase,γ-GCS),免疫组化检测核因子-κBp65片段(p65 subunit of nuclear factor kappa B,NF-κBp65)、核因子-E2相关因子(nuclear factor-E2 related factor,Nrf2),荧光定量PCR方法检测γ-GCSm RNA。结果吸入组大鼠GSH、NF-κBp65下降,γ-GCSm RNA、Nrf2水平升高,6 h组较明显。上述指标吸入3 h组变化不明显。结论低剂量吸入H2S对大鼠肺组织氧化无明显作用,吸入80 ppm H2S 6 h对氧化系统的影响是可能引起肺损伤。

Objective To investigate the different time of hydrogen sulfide inhalation in inhibiting oxidative stress of lung injury in rats. Methods Eighteen male SD rats were randomly allocated into control group, H2S inhalation 3 h and H2S inhalation 6 h group, 6 in each group. There were no H2S inhalation in the control group, while the concentration of glutathione （GSH） in bronchoalveolar lavage fluid （BALF） and the γ -GCS in homogenized lung tissue of rats in the H2S inhalation 3 h, 6 h groups were observed by ELISA assay, the immunohistochemically was applied to detect the relative expression of NF- κBp65 and Nrf2 with Image Pro Plus 6.0 software, and the method of fluorescence quantitative PCR were used to detect the expression of γ-GCSmRNA in homogenized lung tissue of rats. Results Compared with the control group, the GSH index decreased, and the concentration of γ-GCSmRNA and the expression of Nrf2 in the homogenized lung tissue of rats in the 6 h group significantly elevated, while those indexes in the H2S 3 h group did not change significantly. Conclusion Low inhalation of hydrogen sulfide for 3 hours shows no significant effects on the oxidative stress in the lung tissue of rats, while 80 ppm inhalation of hydrogen sulfide for 6 hours can potentially induce oxidative stress and cause damage to lung tissue.