摘要
目的观察沉默信息调节因子1(silent information regulator 1,SIRT1)小干扰RNA(siRNA)对前列腺癌DU145细胞迁移和基质金属蛋白酶中MMP2和MMP9表达变化的影响。方法体外培养DU145细胞,实验分Scramble siRNA组和SIRT1 siRNA组;利用脂质体介导转染技术转染前列腺癌DU145细胞,Western blot方法检测DU145细胞中SIRT1的干涉效能;划痕实验、平板克隆实验和Transwell迁移实验研究SIRT1对其体外迁移运动能力的影响;Western blot方法检测DU145细胞中MMP2和MMP9的蛋白表达。结果与Scramble siRNA组比较,SIRT1 siRNA组SIRT1的蛋白表达水平降低,明显抑制DU145细胞的迁移,降低MMP2和MMP9蛋白。结论下调SIRT1的表达可以抑制前列腺癌细胞DU145的迁移,其机制可能与改变基质金属蛋白酶中MMP2和MMP9的表达相关。
Objective To observe the effects of double-stranded small interfering RNA （siRNA） of silent information regulator 1 （SIRT1） on the cell migration and the expression levels of MMP2 and MMP9 of matrix metalloproteinases in prostate cancer DU145 cells. Methods DU145 cells were cultured in vitro and divided into scramble siRNA group and SIRT1 siRNA group randomly. The efficiency of SIRT1 siRNA was examined by Western blot, and the cell migration of DU145 cells was assayed by wound healing, colony formation and transwell migration experiment. The protein expression of MMP2 and MMP9 were determined by Western blot. Results Compared with scramble siRNA group, the protein expression of SIRT1 in SIRT1 siRNA group decreased, and the colony formation and cell migration of DU145 cells were restrained significantly and the expression of MMP2 and MMP9 also decreased. Conclusion The cell migration of DU145 cells can be inhibited by down-regulating the expression of SIRT1, and its mechanism mav relate to the chan^es of the expression of MMP2 and MMP9 in matrix metallooroteinases.
出处
《解放军医学院学报》
CAS
2014年第12期1253-1256,共4页
Academic Journal of Chinese PLA Medical School
