单核细胞趋化蛋白1及其受体在冠心病猝死中的表达

Expression of Monocyte Chemotactic Protein-1 and Its Receptor in Sudden Coronary Death

目的探讨单核细胞趋化蛋白1(monocyte chemotactic protein-1,MCP-1)及其受体CC类趋化蛋白受体2(CC chemokine receptor-2,CCR-2)在冠心病猝死(sudden coronary death,SCD)和非SCD冠状动脉粥样硬化斑块中的差异表达。方法应用免疫组织化学方法检测MCP-1和CCR-2在SCD组、动脉粥样硬化组(冠状动脉粥样硬化但非SCD)、对照组(冠状动脉正常)的差异表达。结果 MCP-1在三组间的阳性细胞表达率分别是78%、47%、0%,两两比较差异均有统计学意义(P<0.05);CCR-2在三组间的阳性细胞表达率分别是72%、47%、0%,在SCD组和动脉粥样硬化组、SCD组与对照组表达差异均有统计学意义(P<0.05),而在对照组和动脉粥样硬化组差异无统计学意义(P>0.05)。结论冠状动脉粥样硬化斑块中的MCP-1及其受体CCR-2的表达增加与SCD密切相关。

Objective To investigate the expression of monocyte chemotactic protein-1 （MCP-1） and its receptor CC chemokine receptor-2 （CCR-2） in coronary atherosclerosis plaques between sudden coro- nary death （SCD） and non-SCD. Methods The expression levels of MCP-1 and CCR-2 in SCD group, coronary atherosclerosis group （non-SCD）, control group （normal coronary artery） were detected by im- munohistochemistry. Results Positive rates of MCP-1 among the three groups were 78%, 47%, and 0%, respectively, with significant expressing differences between each two groups （P〈0.05）. Positive rates of CCR-2 among three groups were 72%, 47%, and 0%, respectively, with significant expressing differ- ences between the SCD group and coronary atherosclerosis group as well as between the SCD group and control group （P〈0.05）, but with no significant expressing difference between coronary atherosclero- sis group and control group （P〉0.05）. Conclusion Overexpression of MCP-1 and CCR-2 in coronary atherosclerotic plaques is closely correlated with SCD.