金银花中绿原酸和木犀草苷的含量测定

Determination of Chlorogenic Acid and Luteolin-7-O-glucoside in Lonicerae Japonicae Flos

目的：建立同时测定金银花中绿原酸和木犀草苷含量的高效液相色谱方法。方法：以乙腈-四氢呋喃（95∶5）为流动相A,0.4%磷酸为流动相B,采用梯度洗脱,0~8 min为88%,8~10 min为80%,10~25 min为80%~75%;绿原酸和木犀草苷的检测波长分别为327 nm和350 nm;流速为1.0 mL/min,柱温为35℃,进样量为20μL。结果：应用梯度洗脱得到较好的分离效果,绿原酸浓度在10.18~162.88μg/mL范围内呈良好的线性关系（r=0.999 9）;木犀草苷浓度在2.01~20.12μg/mL范围内呈良好的线性关系（r=0.999 7）。结论：该方法准确,操作简便,可用于金银花中绿原酸和木犀草苷含量的同时测定。

Objective ： To establish a HPLC method for simultaneous determination of chlorogenic acid and luteo- lin-7-O-glucoside in Lonicerae daponicae flos. Methods： The mobile phase consisted of acetonitrile-THF （95：5） （A） and 0.1% phosphoric acid（B）with gradient elution, 0 and 8 min was 88%, 8 and l0 min was 80%, and 10 and 25 min was between 80% and 75%. The detection wavelength of chlorogenic acid and luteolin-7-O-glucoside was at 327 nm and 350 nm, respectively. The flow rate was 1.0 mL/min; column temperature was at 35 ~C. Results： A better separating effect was obtained by the HPLC gradient elution method. The linear calibration curve of chlorogenic acid and luteolin- 7-O-glucoside were obtained, that concentration range were between of 10.18 and 162.88 mg/mL（r=0.999 9）, and 2.01 and 20.12 mg/mL0~0.999 7）, respectively. Conclusion： The HPLC method is accurate and simple. It can be used to determine the content of chlorogenic acid and luteolin-7-O-glucoside in Lonicerae Japonicae Flos simultaneously.