期刊文献+

新疆家蚕抗菌肽CecropinXJ对荷瘤小鼠免疫系统的影响 被引量:3

Influence of immune function in tumor-bearing mice by CecropinXJ
下载PDF
导出
摘要 目的:抗菌肽是生物防御系统中产生的一类对抗外源性病原体的肽类物质,是生物体内固有的、非特异性免疫系统的重要组成部分。在动物模型及人体中,免疫系统在控制肿瘤方面具有重要作用。本文通过建立BALB/c荷瘤小鼠模型,探讨新疆家蚕抗菌肽CecropinXJ对人食管癌Eca109细胞荷瘤小鼠是否通过影响机体免疫应答发挥抗肿瘤作用。方法:通过免疫组化和流式细胞术等方法检测免疫应答相关细胞因子和DC表面分子的表达。结果:10 mg/kg CecropinXJ对Eca109细胞移植瘤具有显著的抑制作用。10 mg/kg CecropinXJ给药组肿瘤体积和肿瘤重量与生理盐水对照组相比,具有显著差异(P<0.05)。同时,CecropinXJ可抑制肿瘤组织癌细胞的浸润生长而对小鼠体内脏器无影响。在CecropinXJ抑制肿瘤生长过程中,VEGF和b FGF上调表达。与对照组相比,CecropinXJ对荷瘤小鼠的抑瘤作用不能够显著促进CD4、IL-4和IFN-γ的表达,同时也不涉及脾脏中DCs表面分子的表达(P>0.05),但对TNF-α的表达具有显著的促进作用(P<0.01)。结论:CecropinXJ可以显著抑制荷瘤小鼠体内肿瘤的生长,在该过程中,可能并不通过影响机体免疫应答发挥抗肿瘤作用。 Objective:Antimicrobial peptides ( AMPs ) are peptides generated in the biological defense system against exogenous pathogens ,which is all important constituent of the non-specific immune system.The immune system can react and sometimes play an important role in tumor control both in animal models and in humans.In this study,CecropinXJ were used in the BALB/c Eca109 cells-bearing mice model.To investigate whether CecropinXJ exert anti-tumor efficiency through regulating immune response when treated with tumor-bearing mice.Methods:The expression of cytokines and DC surface molecules were detected by immunohisto-chemistry and Flow cytometry.Results:The experiment of mice showed that CecropinXJ could significantly inhibit the proliferation of transplanted tumors in dose-dependent manner.There were significant difference between control group and 10mg/kg CecropinXJ group on the volume and weight of tumor ( P〈0.05 ).CecropinXJ inhibited Eca 109 cells infiltrating growth in tumor tissue without affecting viscera,and the expressions of VEGF and bFGF proteins were higher than those in the control group .The expression of cytokines and DC surface molecules,the results that,compared with the control group,CecropinXJ could not significantly promote the expression of CD4,IL-4 and IFN-γ,while not involving the expression of surface molecules on DCs in spleen (P〉0.05).In CecropinXJ treatment group,TNF-αlevel in serum was significantly higher than the control group ( P〈0.01 ).Conclusion: CecropinXJ could significantly inhibit tumor growth in Eca109 cells-bearing mice,and might not be affected anti-tumor efficiency through regulating immune response.
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2014年第12期1627-1632,1636,共7页 Chinese Journal of Immunology
基金 新疆维吾尔自治区高技术研究发展计划项目(201110101)
关键词 抗菌肽 细胞因子 免疫应答 抗肿瘤 Antimicrobial peptides Cytokines Immune response Anti-tumor
  • 相关文献

参考文献15

  • 1Roscia (,, Falciani C, Bmcei L, et al. The development of amimierobial pepti(les as new antibacterial drugs [ J ], Curr Protein Pept Sei,2013,14 ( 8 ) :641-649.
  • 2Dunn GP, Bruce AT, lkeda H,et al. Cancer immunoediting: from immumsurw.illance to tumor escape [ J ]. Nat hnnmnol, 2002,3 ( 11 ) :991-998.
  • 3Srisailam S, Kumar TK, Arunkumar AL, et al. Crumpled structure of the custom hydnphobic lyric peptide cecropin B3 [ J]. Eur J Bi,-hem ,2001,268 ( 15 ) :4278-4284.
  • 4Chert Y, Xu X, Hong S, et al. RGD-tachyplesin inhibits tumor growth [ J ]. Cancer Res,2001,61 ( 6 ) :2434-2438.
  • 5Lillard JW Jr, Boyaka PN, Chertov O, et al. Mechanisms for induction of acquired host immunity by neutrophil peptide defensins [ J ]. Proc Natl Acad Sci USA, 1999,96 ( 2 ) :651-656.
  • 6Lala A, Lindemann RA, Miyasaki KT. The differential effects of polymorphonuclear leukocyte secretinn (m human natural killer cell aetivity[ J 1. Oral Microbiol hnmunol, 1992,7 ( 2 ) :89-95.
  • 7Papo N, Shahar M, Eisenbach L, et al. A novel lyric peptide composed of DL-amino acids selectively kills cancer cells in cuhare and in mice [J]. J Biol Chem,2003,278(23) :21018-21023.
  • 8Ma XT, Xu B, An LL,et al. Vaccine with -defensin2-transduced leukemic cells activates innate and adaptive immunity to elicit potent antileukemia responses [ J ]. Cancer Res, 2006,66 ( 2 ) : 1169-1176.
  • 9Zhang K,Lu Q,Zhang Q,et al. Regulation of activities of NK cells and CD4 expression in T cells by truman HNP-I ,-2, and-3 [ J ]. Biochem Biophys Res Commun ,2004,323 ( 2 ) :437444.
  • 10Chen J, Xu XM, Underhill CB, et al. Tachyplesin activates th / classic complement pathway to kill tumor cells [ J ]. Cancer Res2005,65 ( 11 ) :4614-4622.

二级参考文献24

  • 1林振胜.布氏杆菌病对人畜的危害及其预防措施[J].福建畜牧兽医,2002,24(z1). 被引量:9
  • 2Imler J, Hoffmann J A. Toll receptors in innate immunity. Trends Cell Biol, 2001,11:304 - 311.
  • 3Dimopoulos G, Muller H M. Innate immune defense against malaria infection in the mosquito. Curt Opin Immunol, 2001,13:79 - 88.
  • 4Hoffmann J A, Reichhart J M. Drosophila innate immunity: an evolutionary perspective. Nat Immunol, 2002, 3:121 - 126.
  • 5Bomon H G, Hultmark D. Cell-free immunity in insects. Annu Rev Microbiol, 1987, 41 : 103 - 126.
  • 6Marchlni D, Giordano P C, Arnons R, et al. Purification and primary structure of ceratotoxin A and B, two antibacterial peptides from the medfly ceratitis capitata. Insect Biochem Mol Bio1,1993,23 (5):591 - 598.
  • 7Hultmark D, Steiner H, Rasmuson T, et al. Purification and properities of three inducible bactericidal proteins from hemolymph of immunized pupae of Hyalophora cecropia. European Journal of Biochemistry, 1980, 106:7- 16.
  • 8Baman H G, Nilsson I, Rasmuson B. Inducible antibacterial defense system in Drosophila. Nature, 1972,237:232 - 235.
  • 9Gellisson G, Melber K, Sanowicz Z A, et al. Hetrologous gene expression in yeast. Gen Mole Microbiol, 1991, 62:79 - 93.
  • 10Lamberty M, Ades S, Uttenweiler-Joseph S, et al. Isolation from the lepidopteran Heliothis virescens of a novel insect dofensin with potent antifungal activity. J Biol Chem, 1999, 274:9320 - 9326.

共引文献33

同被引文献65

引证文献3

二级引证文献9

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部