电针足三里对术后大鼠腹腔粘连组织血管内皮生长因子表达的影响

Effect of Electro-Acunpunture at Zusanli Point on Expression of VEGF in Postoperative Intra-Abdominal Adhesions Rats

目的:观察电针足三里对术后大鼠腹腔粘连组织血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响,探讨电针足三里发挥抗炎作用的机制。方法:雄性Wistar大鼠40只,随机分为:A组(模型组)、B组(模型+电针足三里组)、C组(模型+电针非穴组)、D组(造模后注射α-银环蛇毒素组)和E组(造模后注射α-银环蛇毒素+电针足三里组),每组8只。采用Chiang方法制作大鼠腹腔粘连模型,B组和E组术后给予持续电针刺激双侧足三里穴1 h,连续3 d;C组采用相同频率和时间刺激非经非穴,D组和E组术毕腹腔注射α银环蛇毒素(α-BGT)。各组大鼠于术后第10天处死,剪取磨损盲肠组织,行VEGF免疫组织化学染色,观察镜下组织VEGF表达情况。结果:术后第10天,各组大鼠剖腹可见均发生腹腔粘连,镜下可见VEGF表达均有不同程度增加;B组粘连组织VEGF表达明显低于其余各组(P<0.01)。与A组比较,C组、D组及E组VEGF表达无明显降低或升高,差异无统计学意义(P>0.05)。结论:电针足三里可有效降低术后腹腔粘连组织VEGF过度表达;阻断胆碱能受体α7亚基后施行电针足三里,组织VEGF表达无明显降低,其发挥抗炎机制与胆碱能抗炎通路有关。

Objective： To observe the effect of electro-acupuncture（ EA） at Zusanli points on vascular endothelial growth factor（ VEGF）of postoperative intra-abdominal adhesions,and to explore its anti-inflammatory pathway. Methods： Forty male Wistar rats were divided into 5 groups（ each = 8） ： Group A（ abdominal adhesions model）,Group B（ abdominal adhesions plus EA）,Group C（ shame acupoint control）,Group D（ abdominal adhesions plus α-bungarotoxin） and Group E（ abdominal adhesions plus EA after α- bungarotoxin）. Animal models of abdominal adhesion were produced by Chiang＇s path. Bilateral Zusanli points and shame acupoints were electro-acupunctured with constant voltage for 1 hour at rats＇ waking. The α-BGT（ 1 μg·kg^-1） was injected into abdominal cavity after the surgery. All the rats were sacrificed on the 10 th day,the the VEGF in tissues were evaluated. Results： The rats were sacrificed on the tenth day,the damaged cecum of abdominal adhesion groups developed obvious edema,and respectively adhered with other tissues; Compared withgroup B,the expression of VEGF was higher（ P〈0. 01）; Compared with group A,group C,group D and group E have no decrease VEGF expression or higher,there was no statistically significant difference（ P〉0. 05）. Conclusion： EA at Zusanli points significantly lowered the expression of VEGF after abdominal adhesion challenge; The activation of cholinergic anti-inflammatory pathway might be one of the mechanisms of EA at Zusanli points exerting the anti-inflammatory.