高效液相色谱紫外检测器测定粮食中的T-2毒素

Determining T-2 toxin in grain by UV detection of the High Performance Liquid Chromatography

目的建立高效液相色谱（HPLC）紫外检测器测定T-2毒素的方法,并评价该方法的可操作性,最终推广到下级单位使用。方法采集哈尔滨市市售大米,经过粉碎后,染毒备用。取染毒后大米粉末,加入乙腈水溶液及正己烷萃取,涡旋混合,离心后,移取己烷层氮气吹干,残渣加入甲醇水溶解进样。色谱条件：采用C18常规反相色谱柱（150 mm×4.6 mm,5μm）,流动相为甲醇∶水（7∶3）,紫外检测波长208 nm,柱温为室温,流速0.5 ml/min。结果 T-2毒素的保留时间为11.6 min;在50~800 ng范围内线性关系良好;样品中T-2毒素的平均回收率为93.00%。结论该方法测定T-2毒素含量,结果准确,可用于粮食中T-2毒素的含量测定。

Objective To establish a new HPLC method by UV detection for the assay of T-2 toxin, and evaluate the method of operation, eventually popularize to the subordinate units. Methods Rice on the market of Harbin city was collected, after crushing and exposing to T-2 toxin, rice was extracted by acetonitrile and hexane, then vortexed and centrifuged, the hexane layer was moved and dried by nitrogen evaporation, dissolved by methanol water, finally analyzed by HPLC. T-2 toxin can be separated on the column of C18 （ 150 mm＊ 4. 6ram, 5 p,m） with the mobile phase methanol-water （7：3 ）. UV detection wavelength was 208 nm. The column temperature was at room temperature. The tow rate was 0.Sml/ min. Results This standard curve was linear in the concentration ranged from 50 to 800 ng. The average rate of recovery was 93.00%. Conclusion The retention time of T-2 toxin was 11.6 minutes. This method can determine T-2 toxin content accurately and can be used for the determination of T-2 toxin in grain.