哺乳期磺胺间甲氧嘧啶暴露对仔鼠生长发育及相关激素的影响

Effect of sulfamonomethoxine exposure during lactation period on growth,development and related hormones of neonatal mice

目的:研究哺乳期母鼠接触磺胺间甲氧嘧啶(SMM)对其仔鼠早期生长发育的影响。方法:24只孕鼠分娩后均分为SMM对照组、低、中、高剂量组,即0、10、50、200 mg/kg,仔鼠出生后第1天开始灌胃暴露直至21天,于第1、7、14、21天称量鼠的体重,第22天断头处死取血清,放射免疫法测量三碘甲腺原氨酸(T3)、甲状腺激素(T4)、胰岛素(INS)、生长激素(GH),酶联免疫法测胰岛素样生长因子1(IGF-1)。结果:各剂量组在出生后第1、7、14和21天仔鼠体重差异均无统计学意义(P>0.05)。与对照组相比,高剂量组第3周体重增长百分比(61.5±6.4)%降低,差异有统计学意义(P<0.05);高剂量组的INS水平(32.84±8.28)μIU/ml升高,差异有统计学意义(P<0.05)。T4、T3、GH和IGF-1各剂量组之间差异无统计学意义(P>0.05)。结论:哺乳期母鼠接触SMM影响了子代小鼠早期的生长发育。

Objective： To research the effect of sulfamonomethoxine （SMM） exposure during lactation period oil growth, develop- ment and related hormones of neonatal mice. Methods： After delivery, 24 female mice were divided into control group, low - dose SMM group （0 mg/kg）, middle -dose SMM group （10 mg/kg） and high- dose SMM group （200 mg/kg） ; the neonatal mice were treated with intragastric administration of SMM from the first day to the 21st day after birth, body weights of the neonatal mice were obtained on the first day, the seventh day, the fourteenth day and the 21st day; the neonatal mice were sacrificed on the 22nd day to obtain serum specimens, ra- dioimmunoassay was used to detect triiodothyronine, thyroxine, insulin and growth hormone; ELISA was used to detect insulin like growth factor 1 （ IGF- 1 ） . Results： There was no statistically significant difference in body weight of the neonatal mice on the the first day, the seventh day, the fourteenth day and the 21st day among low - dose SMM group, middle - dose SMM group and high - dose SMM group （ P 〉 0. 05 ） ; the percentage of weight gain on the third week in high - dose SMM group was （61.5 ± 6. 4） %, which was statistically significantly lower than that in control group （ P 〈 0.05 ） ; the level of insulin in high - dose SMM group was （ 32. 84 ± 8.28 ） μIU/ml, which was sta- tistieally significantly higher than that in control group （ P 〈 0. 05 ） ; there was no statistically significant difference in the levels of triiodothry- onine, thyroxine, growth hormone and IGF- 1 among low- dose SMM group, middle -dose SMM group and high- dose SMM group （P 〉 0. 05 ） . Conclusion： SMM exposure during lactation period has impact on early growth and development of the neonatal mice.