山羊骨髓间充质干细胞的分离培养

Isolation and Culture of Goat Bone Marrow Mesenchymal Stem Cells

旨在对山羊的骨髓间充质干细胞（BMSCs）进行分离培养。将分离得到的BMSCs进行传代培养，采用RT-PCR法检测其干细胞转录因子oct4和sox2基因，以验证其干细胞特性；在此基础上绘制BMSCs的生长曲线，对其生物学特性进行直观了解。结果表明，分离到的山羊BMSCs原代细胞呈现出成纤维样，并能够表达oct4和sox2基因，证明了其干细胞特性；其生长曲线呈“S”型，在1～2 d时为潜伏期，第3天时进入指数生长期，第7天时进入平台期。结果提示，分离得到的细胞具有BMSCs的特性，能够用于后续的相关试验研究。

The aim of this study was to observe the biological characteristics of goat bone marrow mesenchymal stem cells （BMSCs）. Samples were collected from goat and the BMSCs were isolated and cultured. RT-PCR was used to verify the stem cell properties of the isolated cells by detecting the stem cell transcription factor oct4 gene and sox2 gene. Furthermore, the growth curve of the identified BMSCs was drawn. The results showed that the BMSCs exhibited primary fibroblast and were able to express the oct4 gene and sox2 gene, which proved its characteristics of stem cells. The growth curve of BMSCs was shown as ‘S’ type, the 1st and 2nd day when the cells were cultured were the incubation period, the 3rd day was the exponential growth phase, and the growth of the cells entered platform phase after the 7th day. It was indicated that the isolated cells were BMSCs and can be used for the subsequent study.