哈尔滨市新甲型H1N1流感病原学及基因特性研究

Research of etiology and genetic characteristic of new influenza A(H1N1) virus in Harbin

目的研究哈尔滨市新甲型H1N1流感病原学和基因特征,为流感防控提供实验室依据。方法收集哈尔滨市流感样病例标本,进行病毒分离和型别鉴定,选取新甲型H1N1流感流行株进行血凝素基因片断的核苷酸序列测定,构建系统进化树。结果 2009年-2012年哈尔滨市流感流行株分别为新甲型H1N1、季H3、季H1和乙型,2009年-2011年新甲型H1N1流感病毒HA基因核苷酸序列的同源性为97.7%~99.9%,与国际疫苗株的同源性为98.0%~99.4%。结论自2009年以来,哈尔滨市新甲型H1N1与季节性流感病毒交替成为流感优势流行株,其基因序列同源性较高,但随时间推移也在发生变化。序列同源性和进化关系表明疫苗可以给人群提供一定程度的保护。

Objective To study etiology characteristic and antigenic variant of new influenza A（ H1N1） virus in Harbin,and provide the scientific basis for making relevant prevention and control strategy. Methods The samples were collected from patients with Influenza- like illness in hospitals in Harbin,and identified typing for virus isolation. Then representative new influenza A（ H1N1） viruses were determined nucleotide sequence of hemagglutinin genes and phylogenetic tree were constructed.Results The preponderance strains were new influenza A（ H1N1）,seasonal influenza H3,seasonal influenza H1 and influenza B from 2009 to 2012 in Harbin. The sequence homologous rates of new influenza A（ H1N1） virus HA gene nucleotide were97. 7% to 99. 9% from 2009 to 2011. Compared with the vaccine strain recommended by WHO,the sequence homologous rates were 98. 0% to 99. 4%. Conclusion The new influenza A（ H1N1） and seasonal influenza virus alternately into flu strains from 2009 in Harbin. These new influenza A（ H1N1） viruses were highly homologous,but differed to a degree from those strains from 2009. The homology and evolutionary relationships of the gene suggested that vaccine may provide people with a degree of protection from the virus.