河南省2010年-2013年志贺菌群分布及其耐药分析

Analysis of distribution and drug resistance of Shigella in Henan province during 2010-2013

目的了解河南省2010年-2013年志贺菌菌型分布及耐药性情况。方法通过系统生化条鉴别菌株,用血清玻片凝集鉴定菌株,K-B法确定药物敏感性。结果 878株志贺菌分为2群、12种血清型,福氏志贺菌(B群)占76.99%,宋内志贺菌(D群)占23.01%,其中以F2a为优势血清型的福氏志贺菌检出率由2010年的33.76%上升到2013年57.57%,宋内志贺菌分离率由43.46%下降到5.05%。进行药敏试验的325株志贺菌株对氨苄西林、甲氧胺嘧啶、四环素、链霉素、萘啶酸5种抗生素耐药率>95%,80%以上的菌株同时对12种抗生素中的6种以上具有耐药。结论河南省近年来志贺菌流行菌型正在发生改变,对目前常用的抗菌药物表现较高的耐药性,多重耐药现象严重。

Objective To investigate the serotype distribution and drug resistance of Shigella strains from clinical patients in Henan province between 2010- 2013. Methods To identify differential strains with systemic biochemical strip,collect identified strains with serum slide agglutination,and determine the drug sensitivity with K- B method. Results 878 shigella bacteria were divided into two groups,and 12 kinds of serotypes. Shigella flexneri（ B group） accounted for 76. 99%,Shigella sonnei（ D group） occupied 23. 01%. The detection rates of F2 a serum of the main type’s shigella flexneri were 33. 76% in 2010,and increased to 57. 57% in 2013,while the isolation rates of Shigella sonnei was 43. 46% in 2010,reduced to 5. 05% in 2013.The drug resistance rate of 325 strains taking part in drug sensitive test was more than 95% to ampicillin（ AMP）,trimethoprim- pyrimidine（ TMP）,tetracycline（ TE）,streptomycin（ S） and nalidixic acid（ NA）. More than 80% strains were multiple drug resistant to at least 6 of the antibiotics simultaneously. Conclusion The Serotyping of shigella bacteria changed these years in Henan Province,the drug resistance and multidrug- resistance phenomenon of shigella bacteria were very serious.