微波辅助合成分子印迹聚合物用于萃取蜂蜜中的氯霉素

Preparation of Molecularly Imprinted Polymers by Microwave-assisted Polymerization for the Extraction of Chloramphenicol from Honey

采用微波辅助法快速制备以氯霉素为模板分子的分子印迹聚合物.将合成的聚合物作为吸附剂,选择性分离和富集蜂蜜样品中的氯霉素,并结合高效液相色谱-串联质谱法对萃取物进行分析.对合成的分子印迹聚合物进行了表征,考察了聚合物的吸附性能及其选择性,并进行了Scatchard分析.结果表明,合成的聚合物为球形,对氯霉素具有良好的识别能力,最大表观结合量可达0.428 mmol/g.该方法的线性范围为0.5-100 ng/g,相关系数〉0.999,蜂蜜中氯霉素的检出限为0.13 ng/g,6种蜂蜜样品中氯霉素的加标回收率范围为88%-93%.

The chloramphenicol imprinted polymers were prepared by means of microwave-assisted polymeri- zation and applied for the separation and enrichment of chloramphenicol from honey. The polymers were ap- plied as solid-phase extraction materials to extract and clean up the chloramphenicol in honey, followed by the liquid chromatography-tandem mass spectrometry （ LC-MS/MS ） analysis. The morphology of polymers was characterized by scanning electron microscope（ SEM） . The binding property and selectivity of polymers were evaluated and the Scatchard analysis was carried out. The polymers appeared to be spherical in shape and had a good selectivity. The apparent maximum binding amount of the polymers was 427. 7 μmol/g. The detection limit of chloramphenicol was 0. 13 ng/g. The linearity range and correlation coefficient of the method were 0. 5—100 ng/g and 0. 999, respectively. The recoveries of six spiked honey samples were in the range of 88%—93%. The proposed method has been successfully applied for the detection of chloramphenicol from honey.