蜡梅种子休眠原因浅析及赤霉素对种子萌发的影响

Inhibiting Substance and Effect of GA_3 on the Seed Germination of Chimonanthus praecox(L.)Link Seed

以蜡梅种子为试材,酸蚀30min后研究种皮透水性;提取种子萌发抑制物,通过其对拟南芥种子萌发的影响研究萌发抑制物的分布;酸蚀处理后用GA3溶液浸种,研究GA3对蜡梅种子萌发的影响。结果表明：蜡梅种子的种皮透水性较差,严重阻碍种子吸水;种子浸提液抑制拟南芥种子萌发,萌发抑制物主要存在于种仁内;125-500mg/L GA3浸种处理可以打破蜡梅种子的生理休眠,提高种子的发芽势和发芽率;其中,250mg/L GA3浸种6h效果最好;初始萌发时间为5d,发芽势和发芽率分别达41.7%和95.8%,比对照分别提前3d,提高25.23%和91.60%。

Taking Chimonanthus praecox （L.） Link seed as material,the water permeability of seed coat was studied after acid etched 30 minutes, the distribution of germination inhibitors were studied by the effect of extracted germination inhibitors on Arabidopsis＇ seed germination,and the effect of GAa on the seed germination using the wintersweet seeds treated by dense sulfuric acid were researched. The results showed that seed coat had a poor water permeability and hindered water absorption seriously, and seed extracting solutions mainly in kernel inhibited the germination of Arabidopsis＇ seeds. The treatment of 125 mg/L to 500 mg/L GA3 could break the physiological dormancy and improved the germination rate and germination performance of the wintersweet seeds. The effective of soaking in 250 mg/L GA3 for 6 hours was the best, and the time of germination was 3 days earlier than the control and the germination rate and germination energy reached 95.8%, 41.7% respectively, and 25.23%, 91.60M higher than that of the control respectively.