摘要
以长白山区野生水芹为试材,在单因素试验的基础上,通过响应面法优化超声波提取野生水芹总黄酮工艺,并对其抗氧化活性进行考察。结果表明:最佳工艺条件为提取温度70℃、提取功率60W、提取时间9.19min,在此条件下,水芹总黄酮提取率为2.9668mg/g,验证试验得出水芹总黄酮提取率为2.9650mg/g。水芹总黄酮对羟自由基具有较强的清除能力,能很好抑制油脂氧化。利用响应面分析法可优化水芹总黄酮提取工艺,得到理论最佳提取条件,验证结果表明该方法科学、高效。
Taking Oenanthe javanica （BI.） growing in Changbai mountain as material,based on single factor experiments, response surface methodology was used to optimize the ultrasonic-assisted extraction flavonoids,and antioxidant activity of total flavonoids were studied. The results showed that,the optimal extraction conditions were extraction temperature 70℃ ;extraction power 60 W; extraction time 9. 19 minutes. Under these conditions, the predicted and experimental extraction yield of apigenin was 2. 9668 mg/g and 2. 9650 mg/g. Extracted total flavonoids had strong scavenging capacity on hydroxyl free base radicals and could better control oil oxidant. In conclusion, response surface method could provide a reliable approach to find optimal conditions for the extraction apigenin from Oenanthe jawanica. The results suggested that the new method was effective and economical.
出处
《北方园艺》
CAS
北大核心
2015年第1期122-127,共6页
Northern Horticulture
基金
吉林省科技发展计划资助项目(2013419008CB)
吉林省科技发展计划资助项目(20110268)
吉林省教育厅"十二五"科学技术研究资助项目(吉教科合字[2013]第495号)
通化师范学院校级资助项目(201270)
关键词
野生水芹
总黄酮
提取工艺
优化
抗氧化活性
Oenanth e javanica （Bl.） DC.
total flavonoids
extraction process
optimization
antioxidant activity
