中药材丹参根腐病生防菌的分离与鉴定

Isolation and Identification of Traditional Chinese Medicine Salvia miltiorrhiza Bunge Root Rot Biocontrol Agents

以聊城市茬平县杜郎口镇中药材种植基地采集的丹参根际土壤为试材,采用梯度稀释法和平板对峙培养法在丹参根际土壤中筛选对丹参根腐病具有较好抑制作用的生防菌。结果表明:初步筛选出10株具有抑菌活性的有益微生物;进一步通过复筛及其抑菌谱的测定分离出2株抑菌效果较好的菌株,分别为2-1和9-2。通过形态学和分子生物学鉴定,2-1和9-2分别为枯草芽孢杆菌(Bacillus subtilis)、解淀粉芽孢杆菌(Bacillus amyloliquefaciens),该试验可为今后高效生防菌剂的研发奠定基础。

Taking rhizosphene soil of Chinese medicine S. miltiorrhiza Bunge root that from langkou town Chinese herbal medicine planting base in Chiping Connty Liaocheng city as material, used gradient dilution method and dual culture method, better inhibitory biocontrol agents to root rot were selected. The results showed that in the rhizosphere soil of Salvia miltiorrhiza Bunge discovered 10 strains beneficial microorganisms. And through the determination of reduplicative screening and its antibacterial spectrum,isolated 2 baeteriostatie strains： 2-1 and 9-2. With the morphology and molecular biology identification,2-1 and 9-2 were bacillus subtilis and bacillus amyloliquefaciens. This lad a foundation for effective bio-eontrol bacteria agent research and development.